Is it possible to identfy the difference between Methyl alcohol, ethyl alcohol and ethyl acetate extract,if so how?

I am working in the column chromatography for extracting plant extracts in Methyl alcohol, ethyl alcohol and ethyl acetate extracts I got confused with the columns that what column I placed which...

31 July 2020 4,937 3 View

How do we apply a bending stiffness on to a wire model in abaqus?

I was trying to mimic the deflection of a cantilever beam in wire on to a model with a portion of the beam replaced by a spring which provides a bending stiffness. I gave a usual spring with...

01 July 2020 6,869 4 View

How to rectify the"DIsplacement Increment for contact is too big" warning in abaqus?

I am currently modelling a joint of a geodesic dome. I previously ran an analysis only with preload of bolts ignoring all contact surfaces of the shank of the bolt and other geometry . This time...

03 June 2019 4,811 5 View

How to fit multiple curves on same data using the curve fitting toolbox in matlab.?

I have a data set and I want to fit multiple curves (say polynomial of order 4, 5, 6 etc) to the same curve using the cftool. While using curve fitting toolbox, it allows to fit only one type of...

21 December 2018 9,333 1 View

Can chitosan metal (Ag and Au) nano composite encapsulate DNA?

I have done DNA encapsulation using chitosan nano particle. Also I found many referee for that. But i couldn't find any references for encapsulation of DNA with chitosan metal nano composite.

26 October 2017 6,883 3 View

How to get K1 and K2 from J-integral for mixed mode?

17 February 2017 4,486 3 View

Why do damaged cells give high fluorescence intensity with rhodamine 123?

16 March 2016 4,380 2 View

Can someone help me to identify this SAED pattern for silver nanoparticle?

16 December 2015 1,022 6 View

How do you apply a sinusoidal load in ANSYS APDL?

I would like to apply a sinusoidal load to a rectangular plate in ANSYS.

29 July 2014 3,284 4 View

Is there any way to export the finite element meshes created in ANSYS (Mechanical APDL) to ABAQUS?

Ansys to abaqus

08 May 2014 1,327 8 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

How to improve RNA isolation?

Hi, I have been struggling with a weird issue for my RNA samples. I used the Qiagen RNA plus mini kit. For some reason no matter how careful I am with the washes, when I measure the RNA, I get...

22 February 2021 1,286 6 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Hello, how can i find a primer to identify Bacillus Sp. with PCR? Can someone help me with this?

I am looking for PCR primers for bacillus Sp.

15 February 2021 2,552 5 View