Is it possible to identfy the difference between Methyl alcohol, ethyl alcohol and ethyl acetate extract,if so how?

I am working in the column chromatography for extracting plant extracts in Methyl alcohol, ethyl alcohol and ethyl acetate extracts I got confused with the columns that what column I placed which...

31 July 2020 4,937 3 View

How do we apply a bending stiffness on to a wire model in abaqus?

I was trying to mimic the deflection of a cantilever beam in wire on to a model with a portion of the beam replaced by a spring which provides a bending stiffness. I gave a usual spring with...

01 July 2020 6,869 4 View

How to rectify the"DIsplacement Increment for contact is too big" warning in abaqus?

I am currently modelling a joint of a geodesic dome. I previously ran an analysis only with preload of bolts ignoring all contact surfaces of the shank of the bolt and other geometry . This time...

03 June 2019 4,811 5 View

How to fit multiple curves on same data using the curve fitting toolbox in matlab.?

I have a data set and I want to fit multiple curves (say polynomial of order 4, 5, 6 etc) to the same curve using the cftool. While using curve fitting toolbox, it allows to fit only one type of...

21 December 2018 9,333 1 View

Can chitosan metal (Ag and Au) nano composite encapsulate DNA?

I have done DNA encapsulation using chitosan nano particle. Also I found many referee for that. But i couldn't find any references for encapsulation of DNA with chitosan metal nano composite.

26 October 2017 6,883 3 View

How to get K1 and K2 from J-integral for mixed mode?

17 February 2017 4,486 3 View

Why do damaged cells give high fluorescence intensity with rhodamine 123?

16 March 2016 4,380 2 View

Can someone help me to identify this SAED pattern for silver nanoparticle?

16 December 2015 1,022 6 View

How do you apply a sinusoidal load in ANSYS APDL?

I would like to apply a sinusoidal load to a rectangular plate in ANSYS.

29 July 2014 3,284 4 View

Is there any way to export the finite element meshes created in ANSYS (Mechanical APDL) to ABAQUS?

Ansys to abaqus

08 May 2014 1,327 8 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

How to identify what type of fillers are in a polymer?

Hello everyone, I have some PHAs blended with fillers and nucleating agents, yet I don't know what kind of fillers or nucleating agents. The producer has shut down and there's no way to get in...

24 February 2021 1,356 8 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View