I got a case of morquie diseases, I would like to check the electrophrotic pattern of a carbohydrate.
We would need more information:- what type of carbohydrates, in what type of matrix ?
it is a mucopolyssacharide like keratin sulfate and Dermatan Sulfate
Hmm I only have limited experience with GAGs. So then the question will be what do you want to do ? If you want to do a kind of quantitative analysis, then you could acquire the appropriate enzyme and analyse the released oligosaccharides using an appropriate LC method. If you want to make a profile of the intact polysaccharides (assuming they are not still attached to the carrier protein) then probably electrophoresis would be the better approach. You would need to put a fluoresent tag on the reducing end of the polysaccharide and use LIF as a detection method. For more help you might also want to take a look at the Glycoforum pages: http://www.glycoforum.gr.jp/ especially under the proteoglycan section. You may also find some real GAG experts there that may be able to help you or collaborate with you.
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