I'm producing lactone using castor oil as the raw material. In 2 L fermenter scale it works effectively with castor oil. However, I'm having difficulty with the scale up. Let me know your suggestion on this.
The key for fermentation scale up is to keep some key parameters the same or proportional. These important parameters may include mixing times, power to liquid volume ratio, oxygen transfer coefficient etc. The following are some useful article to guide fermentation scale up:
Article Scale-up of Industrial Microbial Processes
https://plygenind.com/how-to-scale-up-fermentation-processes/
Hello Vit Mateju,
Thanks for your response. I am maintaining the constant tip speed and KLa which is equal to the small fermenter. The same results which used to get in small fermenter is not achieving in the pilot scale fermenter. I guess its the issue due to oil as the substrate.
Dear Siva,
Dear
Before designing the scale up, it is above all necessary to master certain parameters in batch mode such as the Kla, the stirring speed, the growth rate, the power consumption...
You also mention that you have determined your Kla and that when you carry out your fermentation in a larger volume your results (yields?) are not equivalent; have you taken into account this equation (very often neglected by the industrial world): v= N*Pi*d where N stirring speed, d: vessel diameter which evolves about the size of your fermentor.
I remain at your disposal to answer or advise you in the engineering of fermentation processes
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