Please suggest the statistical tests to find which changes in habitat features have influenced the change in species abundance?

I have two datasets of abundance count (continuous response variable) and habitat features (continuous explanatory variable). One was collected in the year 2000 and another collected in the year...

03 July 2020 5,185 6 View

Which book is the best to follow for doing qualitative research ?

I am doing social constructionist research on everyday life. There are so many books on qualitative research that it is hard to decide which one is among the top best ones. I am searching for a...

12 May 2020 328 3 View

Can anybody help me with the troubleshooting of trichrome staining of rat uterus?

I am using the standard protocol as given in the Bancroft's book,the color distinction is fine but getting pink blotches all over my tissue section..Have increased washing time in each step and...

22 January 2020 1,471 3 View

Statistics of histology scores?

Hi, (a) I am trying to analyze histological scores of more than 3 groups, each having 6 animals. Which statistical tool is the appropriate one? I checked normality which appears to be of mixed...

16 January 2020 4,661 3 View

Cytotoxicity assay from culture supernatant?

Hi, I was planning to perform a cytotoxcity assay wherein I am planning to use the culture supernatant stored frozen at -80 deg C at different time points. Is there any suggestion with regards to...

18 November 2019 3,724 1 View

Similar FPLC peaks for two different samples. How to proceed for sequence identification?

I ran fplc for ultrafiltered samples of muscle and viscera from a fish. Both peaks were extremely similar. Is it required that this denotes the same protein in both samples? Could someone advise...

14 November 2019 980 3 View

Detection of Rat X and Y chromosomes by PCR?

Hi, I was wondering if anyone has the protocol for rat X and Y chromosome detection by PCR particularly Digital PCR. I want to quantify the actual amount of X and Y chromosome from the same...

14 October 2019 9,257 3 View

RNA stability at different temperature?

Hi I was wondering if any one have checked RNA stailbity at different temperature for subsequent PCR amplification. I want to store my RNA isolated from blood vessels before preparing the...

17 July 2019 3,541 12 View

Macrophage culture from rat?

Hi, I want to isolate and culture macrophages from rats (different sites like bone marrow, peritoneal fluid etc.) Most of the reported literature says to use the supernatant from mouse fibroblast...

11 July 2019 7,314 3 View

Why male Wistar rats are preferred over females in diabetes research?

I am a research scholar , conducting research on antidiabetic potential of herbal compounds. I have found that in vivo testing of anti diabetic drugs are mostly done on male wistar rats. So,...

01 November 2018 2,199 6 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View