Differences of DNA replication and repair between prokaryotes and eukaryotes?

I require differences of DNA replication and repair between prokaryotes and eukaryotes mutually exclusive in both

09 October 2018 198 2 View

In what condition Caspase 3Cleaved would be positive even before Caspase 8?

07 August 2016 7,962 3 View

How to use ImageJ software?

I need to quantify the stained picture using ImageJ please let me know step wise how to make use of it. How can i get the area stained ?

03 April 2016 8,530 3 View

No title

31 December 2015 373 3 View

Can Bio Rad Gel doc be used for visualization of western blot?

31 December 2015 4,356 4 View

What consideration to be taken for choosing endonucleases?

You are given the following assignment by a biotechnology firm. transfer gene A from a mouse chromosome to plasmid pBR322 of Ecoli. What would be your first consideration in choosing in one or...

10 November 2015 2,275 0 View

How to cultivate human PBMC in culture flask?

Can some body provide protocol to cultivate human PBMC in cell culture ?

06 July 2015 7,093 4 View

Can any body suggest how to solubilize bacterial protein extracted by acetone precipitation?

03 April 2015 4,481 4 View

Can anybody provide a protocol for protein extraction of staph aureus bacteria?

Can anybody provide me with the best protocol to be used for protein extraction form staph aureus?

01 February 2015 7,465 3 View

Can I store blotting paper after treating with ECL pierce, in case I want to develop it later on with X ray film?

How long can I wait to develop an x ray film after my blotting paper is treated with ECL pierce (Chemiluminescence).

31 December 2014 9,152 4 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View