I am looking for a IoT simulator ?

I am working on a university project so, I m looking for a good Simulator compatible with LoRa and LoRaWAN protocol to simulate an IoT scenario. Thanks in Advance

20 February 2021 6,352 6 View

What is the Role of temperature In Hummers method for the preparation of graphene oxide?

04 January 2021 5,033 2 View

Any recommendations for a good CRISPR/Cas9 commercial system for functional genomics of fungi/yeast?

I look forward to implying a good CRISPR/Cas9 system with a selective maker for functional genomics of important genes from environmental fungi and yeast.

22 December 2020 1,432 2 View

How to implement LEACH-C (LEACH centralized) algorithm for CH selection in WSNs?

I am currently working on a project that is based on optimizing cluster head (CH) selection for energy-efficient wireless sensor networks (WSNs) using evolutionary search techniques. For the...

11 October 2020 5,814 3 View

What is the suitable statistical analysis to test the relationship between two diseases from 5 different regions?

I have only the total number of cases for each diseases (measured by millions) and I'm trying to find a significance relationship for their coexistence throughout the 5 regions...

24 March 2020 4,397 3 View

What do you reommend as an animal model for anemia?

I am looking for animal models for different types of anemia

23 December 2019 210 6 View

SEM RMSEA Outpot Higher than .08?

Hi all, I tested a factorial model and all the indicators are pretty good except for RMSEA which is .1 Is there a problem in that? Do I have to report it or I can just report other fit...

06 November 2019 8,179 5 View

I am looking for new data set (2017 and more ) for Intrusion detection systems?

like NSL-KDD DATA SET

22 October 2019 5,161 1 View

How should we structure the Sustainability track for MBA program?

I am working as a faculty member who is currently involved in Sustainable Development. Currently, we offer 4 MBA courses in this subject . SUS 7010 Global Principles of Sustainability SUS 7111...

07 October 2019 5,852 10 View

MBA vs Data Analytics? Which one do you prefer?

I have some undergraduate students who are willing to continue their master degrees. MBA is a two year program whereas data analytics is the new hot subject which lasts for only one year. In terms...

07 October 2019 4,650 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View