Kantharaja R.

3 Questions 3 Answers 0 Followers

Questions related from Kantharaja R.

How long do a PCR product viable for sequencing?

I have amplified the fungal ITS gene and because of the current emergency situation, I'm unable to send the PCR product for sequencing. So, I stored them at below -10°C. I want to know How long...

15 July 2020 6,901 24 View

Can a frozen mushroom used for mycelial regeneration?

I have some sporocarps of gilled mushrooms frozen at -20°C and I want to regenerate the mycelia from them. can it be used as inoculum? if YES, Please suggest me available methods to do so.

11 November 2019 6,166 5 View

What happens if I process these PCR products for sequencing?

Hi. I amplified about 750bp of DNA from mushroom samples and got smearing bands in QC. what could be the reason? and what happens if I processed these samples for sequencing?

24 July 2019 3,279 6 View