Is there a specific reason to use the brine shrimp lethality bioassay especially natural plant extracts? Or is there a limitation? I am wondering if I can use this procedure for other substances?
I have novel molecules that possibly have biological activity. I made basic target prediction and docking for some of the target proteins. However, this will be my first experience to build up a...
03 September 2023 1,073 1 View
Hi im curious about how much ascorbic acid should i use to make a stock solution for stardard calibration curve (10,20,40,60,80,100ppm). Is it okay if I want to use gallic acid instead of ascorbic...
22 June 2023 2,937 1 View
Hello, What would be the best methods for comparing three independent studies that used the same measurement scale (25 items Likert scale) and I only have the mean and standard deviation for each...
02 January 2022 6,510 10 View
Hi, I'm working on a project that required me to customize the particle size through a chemical process. Currently, we are implementing the process of slurrying the material in a selected solvent...
01 December 2021 4,279 5 View
A. baumannii is a lactose non-fermenter bacteria, I cultured clinical swab on hiCrome Acinetobacter agar Base, then subculture on MacConkey because this bacteria is lactose non-fermenter, it...
15 April 2021 7,051 2 View
as we Knew, A. baumannii is lactose non-fermenter but when I did the subculture on MacConkey agar it dose not convert to yellow color is there any exception?? or we cant do subculture from...
13 April 2021 2,275 11 View
Hi, does anyone have experience in polymorph research related? I would like to ask the technique or method in order to get a new and stable polymorph. Right now I am just playing around with a...
18 March 2020 9,726 6 View
Hello, I am trying to figure out how can I apply two different design values with maximum and minimum goals in an objective function for an optimization process in ABAQUS. More specifically, I...
22 February 2020 4,697 1 View
We produce liposomes by dissolving lipids (DOPE, DOTAP, CHOLESTEROL, PEG) in tert-butanol and then freeze-drying to form a thin film for re-hydration. Weighing small amounts of lipids and...
24 January 2019 8,528 1 View
I"m induced my rice plant numbers of chromosomes (2n=21) using chemical mutugenesis colchicine to double the number of chromosome of my paddy plant and now i want to use flow cytometry machine to...
22 September 2018 7,649 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...
26 July 2024 7,511 3 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...
21 July 2024 9,914 1 View
Their is new research published in the biological control journal, and I am one of the authors. I want to add it to my account at researchgare. How can I do this?
21 July 2024 7,545 0 View
What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.
21 July 2024 1,554 2 View