Is there a specific reason to use the brine shrimp lethality bioassay especially natural plant extracts? Or is there a limitation? I am wondering if I can use this procedure for other substances?
Hi, does anyone have experience in polymorph research related? I would like to ask the technique or method in order to get a new and stable polymorph. Right now I am just playing around with a...
18 March 2020 9,568 6 View
Hello, I am trying to figure out how can I apply two different design values with maximum and minimum goals in an objective function for an optimization process in ABAQUS. More specifically, I...
22 February 2020 4,535 1 View
I"m induced my rice plant numbers of chromosomes (2n=21) using chemical mutugenesis colchicine to double the number of chromosome of my paddy plant and now i want to use flow cytometry machine to...
22 September 2018 7,531 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
01 March 2021 1,133 1 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...
26 February 2021 7,129 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...
16 February 2021 1,337 2 View
I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...
16 February 2021 4,224 3 View
I would highly appreciate it if my fellow ecologists (biologists) provide their opinion on the thoughts below [esp., shortly tell us which path may be more effective, if they know another way, if...
15 February 2021 3,642 6 View
I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...
14 February 2021 3,142 7 View
I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...
11 February 2021 1,328 3 View