How can I plot survival curves for mixed effects cox models (coxme function)?

I'm working with mixed effects cox models and I can't find the way to produce a "predicted" survival curve. All I've read is about coxph objects but nothing about how to interpret the random...

04 January 2021 2,274 2 View

How to plot 3d surfaces from MD simulations with Gromacs?

Hi all, I'm trying to plot Free Energy Landscapes from Gromacs after using the gmx sham function. After using the command: gmx sham -f PCAplot2d.xvg -notime -ls gibbs1-2.xpm (where...

07 July 2020 858 1 View

Where can I find a dataset about aging and stress of IGBTs modules?

I need a set of data in which an igbt module that drives a three-phase motor has been stressed and aged to predict failures using artificial intelligence techniques.

12 February 2020 5,637 3 View

Why I am getting additional bands in PCR after extracting plasmid from transformants and using M13 primers?

Hi, I have been trying to amplify a segment containing my gene of interest in the TOPO TA vector pCR2.1 using the sequencing primers M13 and M13(-20). However, it seems that I am getting some...

21 March 2019 3,221 3 View

How accurate is to evaluate T-regulatory cells in a tumor infiltrate only by immunohistochemistry of foxp3+ cells?

19 June 2016 1,732 3 View

Did anyone try the lentivirus for CRISPR and nCas9 of abm?

I want to knockout a gen and I would like to know how to select the clones without the protein. There is a antibiotic sistem in the plasmid but that does not provide that the mutation take place...

31 December 2015 9,815 2 View

How I can include metal ions in a enzyme when I am using modeller?

I am relatively new at bioinformatics, and I am trying to model an enzyme for docking analysis. I have used modeller, but the template of my enzyme does not include de metal atoms that I know are...

04 October 2015 4,559 3 View

Is there any reference material about journal clubs in disciplines other than medicine?

I am currently searching for information about the history of Scientific Journal Clubs, but I have only found medical literature. Any suggestion would be welcomed.

03 April 2014 7,482 12 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...

18 February 2021 9,109 5 View

Why are Sanger Sequencing reads interrupted in some edited clones?

I have a Crispr/Cas9 edited bulk population and I can briefly check the indel ratios in the bulk population using Sanger sequencing. After cloning single cells, I wanted to check the indel types...

18 February 2021 8,198 6 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Why do I have trouble with cloning duplicate gene in the same plasmid?

Hello! I am trying to generate a construct in this format: seqA - seqB - linker - seqC - seqB into pSF-CMV-Ub-Puro-Ascl plasmid 0.5kb 2kb 66 0.25kb 2kb I have tried many...

15 February 2021 7,118 3 View