How can I plot survival curves for mixed effects cox models (coxme function)?

I'm working with mixed effects cox models and I can't find the way to produce a "predicted" survival curve. All I've read is about coxph objects but nothing about how to interpret the random...

04 January 2021 2,274 2 View

How to plot 3d surfaces from MD simulations with Gromacs?

Hi all, I'm trying to plot Free Energy Landscapes from Gromacs after using the gmx sham function. After using the command: gmx sham -f PCAplot2d.xvg -notime -ls gibbs1-2.xpm (where...

07 July 2020 858 1 View

Where can I find a dataset about aging and stress of IGBTs modules?

I need a set of data in which an igbt module that drives a three-phase motor has been stressed and aged to predict failures using artificial intelligence techniques.

12 February 2020 5,637 3 View

Why I am getting additional bands in PCR after extracting plasmid from transformants and using M13 primers?

Hi, I have been trying to amplify a segment containing my gene of interest in the TOPO TA vector pCR2.1 using the sequencing primers M13 and M13(-20). However, it seems that I am getting some...

21 March 2019 3,221 3 View

How accurate is to evaluate T-regulatory cells in a tumor infiltrate only by immunohistochemistry of foxp3+ cells?

19 June 2016 1,732 3 View

Best way to check the mutations with CRISPR in clones?

Hi, I have already read about the Surveyor Mutation Detection Kit but if I do with a clon with the mutation in the two alleles I will not obtained two bandes because I dont have any heteroduplex,...

01 February 2016 4,937 1 View

Did anyone try the lentivirus for CRISPR and nCas9 of abm?

I want to knockout a gen and I would like to know how to select the clones without the protein. There is a antibiotic sistem in the plasmid but that does not provide that the mutation take place...

31 December 2015 9,815 2 View

How I can include metal ions in a enzyme when I am using modeller?

I am relatively new at bioinformatics, and I am trying to model an enzyme for docking analysis. I have used modeller, but the template of my enzyme does not include de metal atoms that I know are...

04 October 2015 4,559 3 View

Is there any reference material about journal clubs in disciplines other than medicine?

I am currently searching for information about the history of Scientific Journal Clubs, but I have only found medical literature. Any suggestion would be welcomed.

03 April 2014 7,482 12 View

Why are cells washed with phosphate buffer saline?

Cytoplasmic Immunofluorescence Analysis

05 December 2012 9,321 20 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Your thoughts on the spherical densiometer to estimate relative species canopy cover?

Hi biologists and biogeomorphologists, I want to characterize vegetation plots on a transect that follows a successionnal gradient from a river bed to a mature forest. Ecologically, I am...

26 February 2021 5,720 2 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View

How to Study a proton NMR of Graphene prepared by Electrochemical Exfoliation technique?

I performed a Proton NMR of graphene powder prepared by electrochemical exfoliation technique. It shows some peaks. But I can't understand it.

21 February 2021 8,947 3 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View