Patricia Santofimia-Castaño

2 Questions 1 Answers 0 Followers

Questions related from Patricia Santofimia-Castaño

Best way to check the mutations with CRISPR in clones?

Hi, I have already read about the Surveyor Mutation Detection Kit but if I do with a clon with the mutation in the two alleles I will not obtained two bandes because I dont have any heteroduplex,...

02 February 2016 5,028 1 View

Did anyone try the lentivirus for CRISPR and nCas9 of abm?

I want to knockout a gen and I would like to know how to select the clones without the protein. There is a antibiotic sistem in the plasmid but that does not provide that the mutation take place...

01 January 2016 9,896 2 View