Hi everyone

I’m having a lot of problems trying to characterize  T-regulatory cells  as infiltrates in tumors by FAC`s,  the main problem is that the fixation permeabilization buffers needed for Foxp3, intracellular staining are killing  most of my cells.  So I have seen many papers using Foxp3 marker by IHC but  how accurate is this approach?   Do i have to perform a  triple immunofluorescence using CD4, CD25, Foxp3 in my tissue samples?  Thanks in advance.

Best.

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