Hi everyone
I’m having a lot of problems trying to characterize T-regulatory cells as infiltrates in tumors by FAC`s, the main problem is that the fixation permeabilization buffers needed for Foxp3, intracellular staining are killing most of my cells. So I have seen many papers using Foxp3 marker by IHC but how accurate is this approach? Do i have to perform a triple immunofluorescence using CD4, CD25, Foxp3 in my tissue samples? Thanks in advance.
Best.