I am preparing samples for BigDye sequencing. After PCR, I did ethanol precipitation to clean up the samples. In order to dry up the samples (after removing 70% ethanol), is it okay for me to dry them at 65C about 10min? I am just wondering whether the high temperature will affect the results.
DNA samples are very stable, but you can use 50 ºC to ensure that does not degrade.
Dear Yen,
You can leave the DNA sample at room temperature or laminar air flow for 20 min. Thats enough. If you dry the sample at high temperature it will be difficult to dissolve the pellet in buffer.
Hello Yen! In regards to your question, I think under normal conditions, that air-drying your DNA at room-temperature would be the safest. And if you want it to dry faster, then I wouldn't recommend going beyond 40-45 degrees Celsius, as dsDNA would still be relatively stable at these temperatures, without compromising the solubility of the pellet. In theory, if your sequence has a large number of bp, it should be able to tolerate that much higher temperature without denaturation. The results, otherwise, shouldn't be affected.
It is better to dry at room temperature or in laminar air flow. I usually dry at 37OC so that it dissolves immediately in water or TE buffer.
I usually dry my DNA samples at room temperature in laminar hood. you can try it. Never had problem with my samples. DNA samples always turns up normal!
Best of luck!
You can dry at 55 °C but if you can dry at room temperature without any problem why to take a risk. We usually dried at room temperature under vacuum evaporator.
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