Qudsia Sehar

3 Questions 8 Answers 0 Followers

Questions related from Qudsia Sehar

How to concentrate lentivirus and increase infection efficiency?

Hello there! I have some query to solve regarding my lentivirus infection! I have successfuly generated lentivirus particles by 293T transfection. And i have used this supernatent to infect 293T...

09 September 2017 7,793 8 View

Site directed mutagenesis of 12kb plasmid?

Hello all! I have been trying to mutate 6 nucleotide sequnce (replace) using a pair of primers (sense and antisense, 25 bp long specific hybridization and with overhang (VWG,NSG,NWC) i am using...

06 June 2017 1,133 7 View

Mouse blood serum contamination and degradation at room T?

Hello everyone. I am doing immunization. after doing ELISA, i left serum collected from mice at room temperature for more than 10 hrs. Is there any chance that it is contaminated or my Antibody...

10 October 2016 6,847 5 View