If one of the proteins in S.pombe was tagged with FLAG, is this pombe still considered as wild type?

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Sven Reischauer

No, you created an allele. That said, you should give it an allele number (according the yeast nomencleature) and thats how you will call it.

Martin Klvana

The FLAG-tag is attached to the wild-type sequence; the FLAG-tag does not modify the wild-type sequence. Hence: "FLAG-tagged wild-type protein".

Dominique Liger

Hi there,

it's not wt anymore as it is genetically modified though its phenotype might still be wt.