How to process RNA-seq results?

I have obtained the RNA-seq results, which is in an excel file with all the fpkm values. May I know how do I check the gene expression profiles? Is there any software for this or I gotta manually...

04 May 2018 3,338 9 View

How to determine a protein is working downstream of another protein?

If overexpression of protein A can rescue the growth defect caused by mutation in protein B, can I consider protein A is working downstream of protein B? If yes, why is it so?

04 May 2017 7,522 24 View

Can anyone suggest method to extract membrane proteins?

Currently I am having problems in extracting membrane proteins (ammonium transporters), can anyone help? I used LDS in extraction and I did not heat the samples. Proteins in lane 1-4 seem like...

03 April 2017 773 20 View

Best temperature to dry DNA samples after ethanol precipitation?

I am preparing samples for BigDye sequencing. After PCR, I did ethanol precipitation to clean up the samples. In order to dry up the samples (after removing 70% ethanol), is it okay for me to dry...

09 October 2016 6,100 8 View

Overexpression of a gene is toxic to the cell? Why is it so?

Currently I am working on a new protein in fission yeast. Based on the predicted function, it is involved in transcription. We found that when this gene is overexpressed, the cell growth is...

06 July 2016 8,718 1 View

If one of the proteins in S.pombe was tagged with FLAG, is this pombe still considered as wild type?

One of the proteins in S.pombe that I am working on is tagged with FLAG, so can I still call this strain as wild type as it contains a fragment of foreign DNA? If no, what is the right term for it?

05 June 2016 8,096 3 View

Can I re-digest my digested PCR product?

I have digested my PCR product with NEB Not1 and Nde1 for 1hr. I have cut the band and purified the product. However I afraid the digestion is incomplete, can I re-digest them again? Is there any...

04 May 2016 2,655 17 View

Anyone has protocol for TaKaRa PrimeSTAR mutagenesis basal kit in English?

02 March 2016 4,844 6 View

What are the characteristics of a broad host range plasmid?

May I know what are the characteristics of a BHR plasmid? Is this kind of plasmid occurs naturally or we can try to engineer a narrow host range plasmid to become one?

01 February 2016 7,160 0 View

What is the maximum number of plasmids in a cell?

May I know what is the maximum number of plasmids (with different origins of replication) can be taken up by a cell? What factors affect this? The maximum I have tried was two.

01 February 2016 4,336 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

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19 February 2021 8,647 3 View

How to improve dissolution of neosome?

I have made neosome from herbal drug. Neosomes are stable. But I face problems in dissolution. I used minimum ratio of 1:1:0.25 ( Drug:span 60: Cholesterol and GMs. Now how can I active better...

17 February 2021 3,584 1 View

How to determine the drug loading and the drug release capacity of a polymer micelle using UV spectroscopy?

Currently, I am working on PLA-PEG copolymer micelle for the drug delivery system. I have seen several papers where some researcher talks to centrifuge the drug-loaded micelle to separate the...

13 February 2021 5,822 7 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View