Hi,
Does anyone have any recommendations on what methodology to use for the direct cloning and heterologous expression of a 69kb NRP gene cluster from Gram-positive bacteria?
Thank you!
Article Direct cloning and heterologous expression of the salinomyci...
Please go through the paper.. that might help you
Golden gate! Check the recent paper:
A unified multi-kingdom Golden Gate cloning platform
RecET direct cloning, developed by the team of Pro. You-Ming Zhang in Shandong University, is an efficient method.
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