Lucy Butler

2 Questions 2 Answers 0 Followers

Questions related from Lucy Butler

Best Protocol for Biosynthetic Gene Cluster Heterologous Expression?

Hi, Does anyone have any recommendations on what methodology to use for the direct cloning and heterologous expression of a 69kb NRP gene cluster from Gram-positive bacteria? Thank you!

07 November 2019 3,280 3 View

Designed primers ran on a gel, not working?

Hi, I am looking for advice. I designed my own primers for human TBP, TGFB1 and ITGB6 Gene's. To test my primers I ran a PCR and gel, however, it didn't work. Does anyone have any solutions? I...

21 March 2019 4,048 3 View