Best Method for Quantifying miRNA in Sperm Samples?

More Mitchell Rusten's questions See All

How to model variable thickness composites in LS-DYNA?

I am trying to model a variable thickness composite part in LS-DYNA and I am unsure on how to implement this. I have used PART_COMPOSITE in the past when testing with a composite part with the...

23 April 2024 9,376 1 View

EV Isolation from flash frozen vs fresh skeletal muscle samples?

I am planning to isolate EVs from skeletal muscle samples and see that most studies doing so incubate fresh tissue in media for EV isolation. I am wondering what the drawbacks of using flash...

30 January 2024 7,979 0 View

Current literature on police research studies between 2018-2023?

Issue in addressing police research study gaps in literature to identify job satisfaction or dissatisfaction among police officers. Historical literature on police officer job satisfaction not an...

05 October 2023 5,290 0 View

How do I add supplemental material from a published OA paper ?

20 September 2023 6,335 2 View

How do i make a frog species count using acoustic monitoring?

I am using my smartphone to record the frog calls overnight, How can i tell which species is more dominant from the calls they make?

21 June 2023 8,703 1 View

How to solve fsQCA error in R Studio?

Hey, I am currently writing my Master thesis and I'm conducting an fsQCA using the QCA package in R Studio. In total, I have 9 causal conditions which I all calibrated already. However, I found...

24 May 2023 9,255 0 View

I'm looking for the RAHQ scale by Man & Pakenham?

Hi, I'm trying to find the Responses to Auditory Hallucinations Questionnaire (RAHQ) by Mann & Pakenham. I'm struggling to find the scale and scoring instructions. Does anyone know where I can...

16 November 2022 9,213 2 View

How can I reduce excess weight recovery from microplastics ZnCl2 density separation of low-mass samples?

I am trying to refine a density separation method for quantifying the mass of microplastics in a water sample. I am encountering a persistent problem of recovering excess weight in all my spike...

03 November 2021 3,664 4 View

Is a Chi-square test appropriate for comparing the percent scores of one sample on 2 different tests with different numbers of items?

The mean score was 93% on one test and 88% on the other.

18 October 2021 3,232 5 View

Phosphate free Buffers for SYTOX Green?

Hello! I am trying to use SYTOX green along with the autofluorescence of chlorophyll to determine the viability of micro algae in an Alginate Laponite hydrogel. Unfortunately there are some...

13 July 2021 1,973 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Tomoyasu Sugiyama

You might want to read PMID: 22984523.

Oscar Rafael Escate Chávez

Hello Mitchell

I found a interesting method to help you in this article:

The Bull Sperm MicroRNAome and the Effect of Fescue Toxicosis on Sperm MicroRNA Expression

Heather M. Stowe ,

Samantha M. Calcatera,

Marcy A. Dimmick,

John G. Andrae,

Susan K. Duckett,

Scott L. Pratt

PLOS

Published: December 2, 2014

http://dx.doi.org/10.1371/journal.pone.0113163

Regards