FEA solver as a black box for optimization?

Is there a Python project where a commercial FEA (finite element analysis) package is used to generate input data for a freely available optimizer, such as scipy.optimize, pymoo, pyopt, pyoptsparse?

18 October 2020 3,310 3 View

Can anybody identify and advise on holoparasitic plants living inside cacti?

Four cacti in our conservatory carry holoparasitic plants inside them which have flowered or are still flowering this year. They appear to be two species. Can anybody answer these questions: -...

24 September 2020 9,749 3 View

ANSYS FLUENT is only launching in parallel. Can somebody please help?

My fluent is not launching in series at all, even when I uncheck the parallel option and i also have no option for using series. Please help!!! Many thanks!

04 September 2020 4,199 4 View

I am trying to simulate an electric arc formed because of current flowing through air between 2 conductors in ANSYS Fluent. Can i use MHD module?

If i use the MHD module, i will have to provide the values of external magnetic field, but isn't the magnetic field established as a consequence of the current flowing through the air? How am i...

15 August 2020 200 1 View

After data collection we saw that items were missing in survey, is there any way to fix this?

Unfortunately, we just realized after data collection, when we wanted to start analyzing our data, that some items were missing in the online survey. The survey consisted of three parts (three...

21 April 2020 6,736 11 View

Removal of dead cells before harvesting for Proteomics?

Dear all, I am culturing some suspension cell lines. I am planning to perform proteomics (LC-MS/MS) experiments on them. Unlike adherent cells (viable cells are adhering to the substrate and...

03 March 2020 2,329 3 View

Enrichment of AHA-tagged proteins: Should I use DBCO- or Alkyne-based methods?

Dear colleagues, I would like to seek your kind advice on click chemistry enrichment of azidohomoalanine (AGA)-tagged proteins. From literature, I learned that there are 2 main avenues, either...

18 February 2020 8,725 2 View

Contemporary LGBTQ+ literature/writers in Ecuador?

I am looking to find contemporary (2005 and onward) LGBTQ+ literature/texts (novels, poems, digital literature, correspondence, social media writers) by Ecuadorian writers (preferably LGBTQ+ too,...

04 February 2020 9,780 3 View

Could anyone give me a clue on the identification of this moth?

It has been found feeding on Eucalyptus leaves in Indonesia. Family or genus, maybe? Thank you.

25 December 2019 6,971 3 View

Small scale columns for immobilized enzyme activity screening?

I have been screening the activity of immobilized enzymes in shaken tubes and need to move into testing activity under flow in a column. I am looking for recommendations on what column / pump /...

22 November 2019 6,444 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View