Where can I find Hammerhead Ribozyme sequences?

How do I ensure I correctly add the Hammerhead ribozyme sequence (and the correct one?) on the 5'end of the cDNA plasmid of the viral sequence I want to cleave?

07 December 2020 2,276 1 View

FEA solver as a black box for optimization?

Is there a Python project where a commercial FEA (finite element analysis) package is used to generate input data for a freely available optimizer, such as scipy.optimize, pymoo, pyopt, pyoptsparse?

18 October 2020 3,310 3 View

After data collection we saw that items were missing in survey, is there any way to fix this?

Unfortunately, we just realized after data collection, when we wanted to start analyzing our data, that some items were missing in the online survey. The survey consisted of three parts (three...

21 April 2020 6,736 11 View

Contemporary LGBTQ+ literature/writers in Ecuador?

I am looking to find contemporary (2005 and onward) LGBTQ+ literature/texts (novels, poems, digital literature, correspondence, social media writers) by Ecuadorian writers (preferably LGBTQ+ too,...

04 February 2020 9,780 3 View

Could anyone give me a clue on the identification of this moth?

It has been found feeding on Eucalyptus leaves in Indonesia. Family or genus, maybe? Thank you.

25 December 2019 6,971 3 View

Small scale columns for immobilized enzyme activity screening?

I have been screening the activity of immobilized enzymes in shaken tubes and need to move into testing activity under flow in a column. I am looking for recommendations on what column / pump /...

22 November 2019 6,444 1 View

Who can share a finite element model of the UCF Benchmark structure?

I am looking for a finite element model of the University of Central Florida (UCF) Benchmark Structure in an appropriate format (e.g. SAP2000, Abaqus, Ansys, NASTRAN).

25 September 2019 4,268 1 View

Who knows a good surgical monitoring system for mice?

I am looking for a good surgical monitoring system for mice, mainly ECG recording. For different suppliers it is mostly the same Scintica Rodent Surgical Monitor+ system. Does anyone know a...

28 February 2019 2,000 1 View

What is the composition of Vector M.O.M. Blocking Reagent?

Has anybody an idea what might be in the Vector M.O.M. blocking reagent? The kit works very well in background reduction and one could use rabbit antibodies on rabbit etc. if we would know the...

02 December 2018 7,486 3 View

In vitro cardio toxicity testing?

I'm looking for a reliable cell culture model to test potential cardiotoxicity of a large number of compounds.

26 March 2018 1,715 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

RNA degraded after good nanodrop readings?

23 February 2021 8,850 2 View

Is there a way to extract total RNA and protein from cell culture at the same time, without denaturing protein?

Hi all, Is there a way to extract RNA and protein from the same cell culture sample? Because we need to use the protein for gelatin zymography at later time, we are looking for a method that can...

22 February 2021 5,341 5 View