Fujiyama et al 2019 have shown that use of a cocktail of three enzymes (Collagenase D, Dispase I and DNase I), rather than traditional mechanical grinding, for cell isolation resulted in significant improvement of isolation of macrophage sub-populations, particularly MZMs and MMMs in the spleen, as determined by CD11bhi F4/80medTim4hi and CD11bhi F4/80med Tim4med.

For your ref..

https://academic.oup.com/intimm/article/31/1/51/5106941

BW

For the isolation of macrophages from mouse liver and spleen and then cells were characterized by flow cytometry method is described by Timo ten Hagen et al, in result they were got a high yield of pure Kupffer cells (total of 10 X lob/g liver) and enrichment of splenic macrophages to 20%.

https://core.ac.uk/download/pdf/43314853.pdf

Article The Isolation and Characterization of Murine Macrophages

In second ref; different basic protocols are described for Isolation of macrophages from different organs and yield of macrophages increased by injecting eliciting agents.