Vinod Nadella

5 Questions 107 Answers 0 Followers

Questions related from Vinod Nadella

What is the best way to enhance adhesion of Neutrophils isolated from murine bone marrow?

I am after isolating neutrophils from murine bone marrow using histopaque based density gradient centrifugation. I end up with Ly6G+ neutrophils with 95% viable and 94% purity as assessed by...

19 May 2023 2,577 0 View

Does anyone have expertise isolating macrophages from tissue and culturing them in vitro?

I am interested in culturing macrophages isolated from mice skin tissue and culture them on glass slides for staining and imaging. Do these tissue macrophages adhere in the same fashion as like...

09 November 2021 6,083 1 View

Any leads for medical grade injectable Type 1 collagen for in vivo work?

I am looking for buying injectable Type 1 collagen for in vivo work. I see some like collagen implants, cross-linked forms and mostly with combinations. But I am after a medical grade injectable...

08 June 2021 2,851 0 View

Any reference for Alcian blue staining of the hyaluronic acid in the mice skin sections?

I would like to quantify hyaluronic acid in the skin section of WT vs Mutant mice. Is Alcian blue staining is a good pick? Any recommendations are appreciated. Thanks

22 April 2021 5,374 1 View

Can ImageJ software be used to quantify collagen from Masson's-Trichrome stained images?

I am after quantifying the blue stained collagen in a Masson's-Trichrome stained skin section from an immune deficient mice and compared the same with its WT control. I am curious to know can...

18 February 2021 858 3 View