1. We have extracted DNA from nasal swabs and BAL using the Qiagen DNA Blood and Tissue Kit for extraction. There is now a Qiagen QIAamp DNA Microbiome Kit available. Has anyone compared DNA yields with the DNA microbiome kit versus the Blood and Tissue kit?
In this paper (Validation of a fast and low-cost alkaline lysis method for gDNA extraction in a pharmacogenetic context; Ruth Ramos et al,. 2014) explain a low cost method for gDNA extraction. I have tried this method and I obtained good yield in the quantity of the gDNA.
for microbiome DNA extraction from various swabs- check PureLink microbiome DNA purification kit (Invitrogen); has a bead beating step. for human DNA isolation from swabs, esp in high throughput samples- MagMax kit
Hi,
You can get better results with QIAamp DNA Microbiome Kit than Qiagen DNA Blood and Tissue Kit. This kit is specialized for swabs and body fluids. Also, in our experience, quality of DNA with spin column technology higher than bead–based technology.
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