Cause of slow bromophenol blue migration and smearing while running SDS PAGE?

While running gels for western blotting, I am currently getting, what seems to be, the blue dye from my sample buffer migrating more slowly in sample lanes leaving blue streaks. These transfer to...

08 February 2021 5,317 3 View

I am proposing to write on effect of stigmatisation and mental illness among BME in Uk. How can I rephrase this pls? Any material on this pls?

I am proposing to write on Stigmatisation and mental illness among the Black and Ethnic Minorities living in the Uk , mostly the Afro-Carribeans. I would like to know how I can re-phrame the...

17 August 2020 3,809 8 View

My logcombiner doesn't work, either version within BEAST 2.5 or 2.6! Any ideas for an online platform, or perhaps a package in R?

I am trying to combine two runs from BEAST 2.6 and strangely my Logcombiner doesn't open. Surely there must be another package that can perform the same function? I have looked for earlier...

19 March 2020 7,701 3 View

What is the rationale of heart rate and temperature monitoring with respect to internet of things?

The relationship that exist between the heart rate ,temperature and internet of things

07 November 2019 7,690 3 View

What caused a western blot to show total protein even after stripping and blocking?

I just finished up a western blot with two specific antibodies (one of which was for tubulin) where, when reading on a 700/800 fluorescent Odyssey scanner, my results look the same as my Coomassie...

03 May 2019 6,286 3 View

XRD sin2Psi stress measurment. Why Sin2Psi=0 value does not match linear d-spacing behaviour?

31 March 2019 9,345 5 View

How can I further reduce formation of dimerisation/oligomerisation of GPCRs during my Western Blot?

Hello all, I am attempting to compare degradation of a GPCR, specifically the Dopamine D2 receptor, with a mutant form of the receptor I have created, with extended agonist treatment. Both...

24 March 2019 1,058 3 View

How do I respond to full-text requests without posting publicly for everyone?

I received a full-text request through RG and clicked the link from the RG email and it brought me to a prompt where I was forced to either agree to publicly post the paper for everyone to see, or...

21 March 2019 3,017 3 View

How can we measure postural sway with two force plates combined?

We would like two asses postural sway using two Kistler force plates combined, so that the participant stays with one foot on each force plate. We need some advice on how to adapt our Matlab...

05 March 2019 3,602 3 View

How do I unscubscribe?

This is not what I thought Research Gate was. I want to unsubscribe since I have no time for social media. Cheryl

14 February 2019 9,401 1 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View