Cause of slow bromophenol blue migration and smearing while running SDS PAGE?

While running gels for western blotting, I am currently getting, what seems to be, the blue dye from my sample buffer migrating more slowly in sample lanes leaving blue streaks. These transfer to...

08 February 2021 5,317 3 View

I am proposing to write on effect of stigmatisation and mental illness among BME in Uk. How can I rephrase this pls? Any material on this pls?

I am proposing to write on Stigmatisation and mental illness among the Black and Ethnic Minorities living in the Uk , mostly the Afro-Carribeans. I would like to know how I can re-phrame the...

17 August 2020 3,809 8 View

Species identification from images: Flying fish, cephapods and jellyfish?

Hello there, We have a number of images taken at the surface and during blackwater dives during an expedition to the Sargasso Sea, August 2019. I am seeking experts that might be able to identify...

06 July 2020 4,958 4 View

What is the rationale of heart rate and temperature monitoring with respect to internet of things?

The relationship that exist between the heart rate ,temperature and internet of things

07 November 2019 7,690 3 View

What caused a western blot to show total protein even after stripping and blocking?

I just finished up a western blot with two specific antibodies (one of which was for tubulin) where, when reading on a 700/800 fluorescent Odyssey scanner, my results look the same as my Coomassie...

03 May 2019 6,286 3 View

XRD sin2Psi stress measurment. Why Sin2Psi=0 value does not match linear d-spacing behaviour?

31 March 2019 9,345 5 View

How can I further reduce formation of dimerisation/oligomerisation of GPCRs during my Western Blot?

Hello all, I am attempting to compare degradation of a GPCR, specifically the Dopamine D2 receptor, with a mutant form of the receptor I have created, with extended agonist treatment. Both...

24 March 2019 1,058 3 View

How do I respond to full-text requests without posting publicly for everyone?

I received a full-text request through RG and clicked the link from the RG email and it brought me to a prompt where I was forced to either agree to publicly post the paper for everyone to see, or...

21 March 2019 3,017 3 View

How can we measure postural sway with two force plates combined?

We would like two asses postural sway using two Kistler force plates combined, so that the participant stays with one foot on each force plate. We need some advice on how to adapt our Matlab...

05 March 2019 3,602 3 View

How do I unscubscribe?

This is not what I thought Research Gate was. I want to unsubscribe since I have no time for social media. Cheryl

14 February 2019 9,401 1 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View