What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How much cell pressure and back pressure should I apply to start for triaxial test for different types of soil?

We have recently purchased a triaxial test instrument (MATEST) for testing different types of soil. After installing software, we are facing a issue of saturation where the samples does not...

23 February 2021 7,194 4 View

Not able to charge-discharge Li-ion coin cell at higher C rate ?

I am working on cathode material. I have made different Li-ion coin cell against Li metal using commercial available cathode materials such as LCO,LMO, LFP and NMC and also with the lab...

18 February 2021 1,450 3 View

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The Folin-Ciocalteu method is an electron transfer based assay, and gives reducing capacity, which is usually expressed as phenolic content. Can anyone share any reference paper where total...

13 February 2021 3,782 3 View

Is it possible to find out the wettability and contact angle of water on the rough or corrugated substrate?

First of all, whether it is possible to identify the presence of water and its amount on the Silicon substrate by using only FT-IR spectroscopy. Can ATR-FTIR spectroscopy find out the wettability...

03 February 2021 9,085 4 View

Following is XRD of iron oxide nano material synthesised using bipyridine derivatives. What are the reasons for Broad Humps?

Even after few repetitions..I am getting similar results. Is is due to organic components?

20 January 2021 9,279 6 View

Spatial thinking and geographical reasoning: what are their conceptual differences and interrelations in teaching geography?

I am conducting a state-of-the-art analysis of these concepts. If you have contributions, please, send me.

17 January 2021 1,123 3 View

Multicollinearity between bioclimatic variables in spatial distribution modelling?

Is there any manual guide to perform multicollinearity test between environmental (19 Bioclimatic) variables for MaxEnt modelling? Using all variables without removing the correlated one affects...

14 January 2021 3,807 7 View

Please share some research papers on the synthesis of Mn3O4 nanoparticles via Hydrothermal method by using Manganese(II) nitrate hydrate?

05 January 2021 9,040 4 View

Why there is need to calculate the chlorophyll content and chlorophyll index. What protocol should I used for calculating chlorphyll in mustard?

I want to know the best and effective method/ protocol to calculate the chlorophyll from mustard plants. Please suggest some relevant and the methods or protocols from which I can easily calculate...

05 January 2021 9,882 3 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

How can you adjust pH of SM buffer?

Good afternoon, please how can you adjust pH of phage SM buffer? I presume that main ingredient that has influence of pH is Tris/HCl buffer, but according to literature its range of pH is from 7...

27 January 2021 5,767 3 View

What is the composition of TM buffer used for concentrating bacteriophage?

Hello everyone, I have to isolate DNA from Brucella phage , but before that, I have to concentrate the phage using PEG 8000. I have got protocol for concentrating the phage using PEG and TM buffer...

24 January 2021 2,767 6 View

How can I transform 50 kb plasmid into ecoli?

I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...

22 January 2021 8,612 3 View

Bacteriophages very effective against infections. Some bacteriophages can be oncolytic and effective against cancers. How B_P recognize cancer cells?

Bacteriophages very effective against infections. Many of them such as anti-Staphylococcus or anti-Streptococcal widely used in practical medicine. Some bacteriophages can be oncolytic and...

03 January 2021 4,769 1 View

How to choose the best segmentation model using the area under the precision recall curve, IOU and Dice metrics?

I am using several U-Net variants for a medical image segmentation task. I get the following values for the performance measures including Dice, IOU, Area under receiver-operating characteristic...

27 December 2020 7,273 3 View

Should i add ATG in the beginning of my polypetide sequence when i want to insert it in a phagemid?

Hi I want to make a recombinant m13 phage displaying my peptide as a fusion peptide on its surface pVIII. Should i add ATG at the beginning when i want to insert it in phagmid vector? Previously,...

18 December 2020 9,931 2 View

Does anyone experience E coli lysis while seed culture?

Hi, I am currently working with BL21(DE3)PLySs and PRSET A vector. Out lab frequently transformation and culture E Coli for protein purification. But recently, even though there is colony after...

13 December 2020 9,167 9 View

I alway get serval big plaques even with very high concentration of bacteriophages. how to get well-distributed plaques with double layer agar method?

I used MS2 (ATCC 15597-B1) strain to do my experiment. I always get serval big plaques (like the picture I attached), but not a plate which has a lot of small plaques I saw from other research....

15 November 2020 7,488 4 View

What are measurement criteria of bacteriophages in TEM pictures?

I've got the TEM pictures of my purified bacteriophage whose host is S. aureus. there are various shapes with tail/tailless (maybe a little tail). in order to place it in a family, I need the...

15 October 2020 5,261 5 View