Atif Khan

5 Questions 22 Answers 0 Followers

Questions related from Atif Khan

How to isolate bacteriophages against Acidothiobacillus thiooxidans?

I have isolated Acidothiobacillus thiooxidans from environmental rust samples using ATCC 9K media. The pH of 9K media varies between 2.5-3.0, making a highly acidic environment for bacteria to...

12 March 2024 7,601 2 View

Why my phages are not showing plaque in plaque assay, but they are showing plaques in spot test of phages on same host?

I have isolated bacteriophages from river water and it is showing complete lysis when it is spotted on the lawn of bacterial host isolated from the same water sample. But, when plaque assay is...

21 February 2020 2,650 5 View

How to precipitate out phages from lysate using polyethylene glycol?

I am working on isolation of phages from sea water. I have isolated phages and kept in oxalate-EDTA buffer. Since, suspension has unknown phages, I am infecting this suspension with bacterial...

08 June 2018 7,241 2 View

How to isolate viruses / phages from sea water samples ?

I have to isolate phages from sea water samples obtained from nuclear power plant to study the thermal ecology aspects of phage infection to their host.

28 May 2018 6,772 2 View

How to plate a particular number of cells at a density of 70% ?

I want to do plaque assay for which I need host cells in a 6-well culture plate. The protocol requires to plate 293 cells at a density of 70%. How to calculate this density percentage ??

16 April 2018 672 2 View