Sakshi Pandey

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Questions related from Sakshi Pandey

Challenges in Stabilizing and Concentrating a Highly Unstable Acetyltransferase for Crystallization?

I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...

20 June 2024 2,552 0 View

Bacteriophage Sequencing by ngs

To prepare bacteriophage samples for sequencing with minimal risk of contamination by other phages or bacteria, I employed PEGNaCl precipitation followed by CsCl ultracentrifugation. However, I...

01 January 1970 1,877 1 View

Bacteriophage infecting a bacterium with an integrated prophage

I've isolated a phage against Staphylococcus aureus, but later discovered that the bacterium hosts a prophage within its genome. Despite this, my phage remains capable of infecting the bacterium....

01 January 1970 3,954 3 View

Bacteriophage plaque size

I've been attempting to isolate bacteriophages from water samples, but unlike my usual observations, this particular phage is forming very minute plaques. Could this indicate something?...

01 January 1970 564 8 View

Challenges in Stabilizing and Concentrating a Highly Unstable Acetyltransferase for Crystallization?

I am working on an acetyltransferase that is highly unstable. Its pI is 6.65, and its molecular weight is around 18 kDa. The protein elutes at 1M imidazole and begins to precipitate immediately...

01 January 1970 9,230 5 View