FAME CG analysis (derivation) ?

29 October 2017 9,586 3 View

Where can I buy the standards of quercetin and gallic acid for a thesis?

02 July 2014 4,690 3 View

Does anyone know about the Sevag method for protein removal?

I am extracting Lentinan from Shiitake and the method says that I need to remove protein usin the Sevag method. I have searched and the only thing that I have found is the usage of chloroform and...

03 December 2012 5,187 5 View

How many people/families currently work on informal e-Waste recycling in the world?

A lot of papers and reports suggests that thousands or millions of people or families work as informal "recyclers" in the world, or at least in developing countries. Nevertheless, it is not clear...

01 January 1970 4,906 3 View

*WESTERN BLOT* How do you transfer high and low MW proteins at the same time? I'm trying to get Claudin-5 (~22kDa) and ZO-1 (~220kDa)

I run the electrophoresis in a pre-cast 4-20% gel at 200 V for ~45 min, then i've tried to transfer them to nitrocellulose membrane in the following conditions (always in a cold, wet environment):...

01 January 1970 7,229 6 View

How many people/families currently work on informal e-Waste recycling in the world?

A lot of papers and reports suggests that thousands or millions of people or families work as informal "recyclers" in the world, or at least in developing countries. Nevertheless, it is not clear...

01 January 1970 502 3 View

Non separation of bands in SDS-page?

Hi all, I'm working on bacteriocine! My proteins don't want to seperate, i m trying 120V for 4 hours, and 7.5% is the concentration of my concentration gel. Do you have any suggestions for me to...

12 November 2020 2,300 5 View

Bacteriocin producing LAB?

Hello Dear scientists, I am doing Bacteriocin related research, I did a lot of activities to isolates Bacteriocin producing LAB and I used NaOH, catalase( Sodium thiosulfate as catalase)...

22 August 2020 3,097 3 View

Replacement of MTT OR TTC?

Hello Dear scientists, is that possible to use DMSO instead of MTT or TTC to evaluate the antimicrobial activity of Bacteriocin in broth?

09 August 2020 3,928 2 View

How can I extract S-layer proteins?

Dear researchers, I need some advice from more experienced people, please. I have a bacterial strain which is producing both S-layer proteins and a bacteriocin with similar MW. How can I...

10 November 2019 1,373 3 View

How can I extract S-layer proteins?

Dear researchers, I need some advice from more experienced people, please. I have a bacterial strain which is producing both S-layer proteins and a bacteriocin with similar MW. How can I...

05 November 2019 4,059 3 View

Can monomeric protein form dimer during protein expression?

I have express protein with nisin expression system, which is of 37 kDa. but i got 74 k Da band in SDS PAGE which is twice then my protein of interest.which is unlikely possible in denaturing...

09 October 2019 8,724 8 View

Can you give us your opinion on optimizing bacteriocin production?

Bacteriocin production is being optimized. However, pH, agitation rate, temperature and time all proceeded under the same conditions. In addition, the condition for growing the bacteria was that...

14 March 2019 6,851 4 View

Can you give us your opinion on optimizing bacteriocin production?

Bacteriocin production is being optimized. However, pH, agitation rate, temperature and time all proceeded under the same conditions. In addition, the condition for growing the bacteria was that...

02 March 2019 3,622 5 View

Ph mediated cell adsorption -desortion bacteriocin?

i want a simple technique for extraction of bacteriocin using ph mediated cell adsorption desorption method for food application also in the end we dissolve in acidified nacl , how can i get red...

31 December 2018 9,411 4 View

Please can you tell me my Bacteriocin SDS-PAGE problem?

I did Tris-Glycine SDS-PAGE. I made this using the methods outlined in the molecular cloning book. but I couldn't see the straight band. What is a problem? I want to get straight band..

17 August 2018 7,065 4 View