Can we use a compensation matrix with single staining that the negative population for each single staining is below zero?

Recently we used single staining to make a compensation matrix. But, for each single staining we saw that 13 to 25 percent of our negative population had been shown below Zero. In this situation...

21 February 2021 9,982 2 View

What is the best studying timeline after injection of B16-F10 melanoma metastases cell line?

I want to inject B16F10 cells into C57BL/6 mice as a melanoma metastases model to study the speed of metastases in different organs. I need advice about the designing of the best timeline for...

14 January 2021 3,704 1 View

How to change the atom numbers of a residue in a pdb file?

I am trying to dock 2 proteins in HADDOCK server, but it repeatedly shows the following Error for the second pdb file: " Error in PDB file. Your PDB contains multiple residues with number 1 in...

25 November 2020 4,753 1 View

From where I can get hourly precipitable water vapor (PWV) data for a location?

Hello, For completing my model for radiative sky cooling, I need the precipitable water vapor (PWV) data for a particular location in order to estimate the atmospheric emissivity. Unfortunately, I...

14 September 2020 343 7 View

I am looking for a job as a solid state chemistry researcher in Germany. Could anybody help me in this regard, please?

I'm PhD graduated in Inorganic Chemistry. Currently I am searching for a new position in metal-organic frameworks (MOFs) & li-ion batteries as a Postdoc or Ph.D. researcher. You can find my...

05 April 2019 2,541 1 View

Which image processing software is the best for gel densitometry?

I am using the "ImageJ" software in order to quantify the protein bands on the SDS-PAGE, but I'm not sure it's the best software for this purpose. Does anyone have the experience of using other...

10 January 2019 2,882 12 View

How to analyze SRB ( Sulforhodamine B ) assay results?

I am working on melanoma B16-F10 cell line and after treatment whit different concentration of drug I do SRB protocol then I read their absorb at wavelengths of 545 nm. now, how to analyze these...

12 December 2018 9,843 2 View

What is the best method to dilute DNA samples?

I want to create a serial dilution of genomic DNA and plasmid samples for absolute qPCR. In order to get repeatable results in the Real time PCR, I want to do a very accurate dilution. I know that...

25 October 2018 5,282 12 View

Does anybody have a Matlab code for LDPC encodong and decoding for a rate other than 1/2?

Hi, does anybody have a matlab code for LDPC encodong and decoding for a rate other than 1/2? My message length is not very large.

05 August 2018 9,338 3 View

Why rust artificial inoculation did not work ?

20 May 2018 4,548 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

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23 February 2021 4,014 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

Can we remove the fmoc group after peptide cleavage?

I have synthesized the peptide using Fmoc based solid phase peptide synthesis. Also I have cleaved the peptide from the resin and lyophilized it, but later realized that, I forgot to deprotect the...

21 February 2021 4,537 3 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How to assing secondary structure to calculated peptide/protein having xyz file?

Hi, I have an optimized structure of some peptides and I would like to represent it as a secondary structure - cartoon. Ok, so server apps ask for pdb file (like Stride). I tried in different...

04 February 2021 517 3 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View