How can I prevent bubbles from forming under a coverslip?

My colleague and I are having some trouble mounting 70 micrometer mouse brain slices. When we initially position the coverslip over the mounted slices, there are no bubbles but when we come back...

10 February 2021 3,580 1 View

What are possible impacts of Virtual Reality Glass (vive pro) on EEG signals?

Hi All I am conducting an experiment using Vive pro Virtual Reality Glass and EEG simultaneously. I figured out that there is a 20 Hz activity in the power spectrum density of EEG signals while...

12 November 2020 4,180 4 View

Can anyone suggest why my PCR results gave big differences in the control group?

I did RT-PCR using SYBR Green and used 2 ul of cDNA per sample to investigate the effect of LPS in primary osteoclast culture. I loaded the same amount of RNA to make cDNA (550ng/sample). However...

17 September 2020 9,193 3 View

Does anyone have a recommendation for a book about Raman related to carbon besides Dresselhaus?

I need more than specifically graphene-related raman. I would like a book more related to changes in various carbon-chains. Thank you.

26 August 2020 5,101 3 View

Hi, can anyone clear up about the molarity concept?

I'm required to prepare 2mM of LiCl for my DNA extraction method, which I calculated to be 0.0212 g of LiCl in 250 ml of water. My supervisor told me to use any volume as long as it is 2 mM....

14 February 2020 9,808 3 View

Any idea on how to make an intractive chromosome Ideograms?

I am trying to make a chromosome ideogram (map genes on a chromosome) in an interactive way; if you click on a section of the chromosome, it expands and shows the corresponding genes in that region.

27 January 2019 9,858 3 View

I would like to find out why QGIS to Web is not used in academic research of Web GIS?

I would like to find out why QGIS to Web is not used in academic research of Web GIS?

15 May 2018 9,351 3 View

About online business acceptance journals?

Hi, Can someone please recommend what ISI or Scopus indexed journals that I can submit my manuscript ? my study is about what is people perception/acceptance of online business in asia? whether...

31 January 2018 7,181 7 View

How can we speed up the time of publishing students work, without turn a frustration for their career? Is it getting worse?

Some data suggest that wait times have increased within certain subsets of journals, such as popular open-access ones and some of the most sought-after titles. At Nature, the median review time...

10 February 2016 7,715 9 View

Where can i find Reves (1983) What makes a good language learner?

I'm looking for an often cited work in the field of Foreign language aptitude research, but which doesn't seem to be available (easily) online: Reves. T. (1983). What makes a good language...

01 January 1970 7,165 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View