What is the use of whole genome shotgun sequencing ?

09 October 2014 8,964 1 View

Can anyone explain the antagonistic activity of bacteria?

When we screen for bio-active compounds producing bacteria we do antagonistic activity. I would like to ask what is the mechanism involved in screening potential bacteria.

09 October 2013 2,662 0 View

Binding capacity of protein?

No interactions but has binding capacity with protein. I have done docking studies and even wet lab experiment . The wet lab experiments showed a good results and not in docking studies like no...

08 September 2013 4,281 3 View

Homology modeling

I have a protein with no X ray crystallography structure in PDB database. I am trying to do homology modeling, I found it is showing only 24% with one protein and few are with 30%. My query is,...

08 September 2012 4,809 18 View

Pass predictions

Can any one tell me interpretation of Pass predictions?

08 September 2012 9,269 0 View

RCSB subbmission

A sequence which was retrieved from NCBI - I have modeled the protein structure and now I want to submit the protein structure to PDB (RCSB). Is it possible to submit in RCSD database? Or can only...

07 August 2012 195 7 View

Servers for molecular docking

Do we have any on-line servers for docking studies. Please suggest some good on-line servers for protein and ligand minimization.

05 June 2012 5,811 13 View

Homology modelling of proteins

I have some questions regarding homology modelling i.e, how can I find the protein sequence ,which as already has a structure in PDB?

03 April 2012 1,618 17 View

Taxonomy of vibrionaceae

Can any one help me how to find the complete taxonomy of the vibrionaceae till date any tools in bioinformatics

31 December 2011 5,966 6 View

Brine Shrimp Lethality Assay

Hi every one I want to test the toxicity test of microbial pure compounds and proceed for anticancer activity in cell lines.Can any one tell me BSL (Brine Shrimp Lethality Assay) is good and...

11 December 2011 2,151 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View