Can anyone explain the antagonistic activity of bacteria?

When we screen for bio-active compounds producing bacteria we do antagonistic activity. I would like to ask what is the mechanism involved in screening potential bacteria.

09 October 2013 2,662 0 View

Binding capacity of protein?

No interactions but has binding capacity with protein. I have done docking studies and even wet lab experiment . The wet lab experiments showed a good results and not in docking studies like no...

08 September 2013 4,281 3 View

Bacterial-type asparaginases Primers

To amplify the bacterial L-asperginases gene, I need the Primers. Can anyone help me to find a suitable primer sequence and related papers .

10 November 2012 2,156 3 View

Homology modeling

I have a protein with no X ray crystallography structure in PDB database. I am trying to do homology modeling, I found it is showing only 24% with one protein and few are with 30%. My query is,...

08 September 2012 4,809 18 View

Pass predictions

Can any one tell me interpretation of Pass predictions?

08 September 2012 9,269 0 View

RCSB subbmission

A sequence which was retrieved from NCBI - I have modeled the protein structure and now I want to submit the protein structure to PDB (RCSB). Is it possible to submit in RCSD database? Or can only...

07 August 2012 195 7 View

Servers for molecular docking

Do we have any on-line servers for docking studies. Please suggest some good on-line servers for protein and ligand minimization.

05 June 2012 5,811 13 View

Homology modelling of proteins

I have some questions regarding homology modelling i.e, how can I find the protein sequence ,which as already has a structure in PDB?

03 April 2012 1,618 17 View

Taxonomy of vibrionaceae

Can any one help me how to find the complete taxonomy of the vibrionaceae till date any tools in bioinformatics

31 December 2011 5,966 6 View

Brine Shrimp Lethality Assay

Hi every one I want to test the toxicity test of microbial pure compounds and proceed for anticancer activity in cell lines.Can any one tell me BSL (Brine Shrimp Lethality Assay) is good and...

11 December 2011 2,151 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

Repository for capture-seq raw reads?

I am a research tech in a wildlife genetics group and my PI and I are trying to figure out how/where to put raw reads in a repository. We have capture-seq NGS from Rapid Genomics. The post-doc who...

17 February 2021 7,575 2 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

No title

17 February 2021 4,217 3 View