When we screen for bio-active compounds producing bacteria we do antagonistic activity. I would like to ask what is the mechanism involved in screening potential bacteria.
Dear all Can you please clarify "what is the use of whole genome shotgun sequencing"
09 October 2014 9,043 1 View
No interactions but has binding capacity with protein. I have done docking studies and even wet lab experiment . The wet lab experiments showed a good results and not in docking studies like no...
08 September 2013 4,351 3 View
To amplify the bacterial L-asperginases gene, I need the Primers. Can anyone help me to find a suitable primer sequence and related papers .
10 November 2012 2,238 3 View
I have a protein with no X ray crystallography structure in PDB database. I am trying to do homology modeling, I found it is showing only 24% with one protein and few are with 30%. My query is,...
08 September 2012 4,878 18 View
Can any one tell me interpretation of Pass predictions?
08 September 2012 9,343 0 View
A sequence which was retrieved from NCBI - I have modeled the protein structure and now I want to submit the protein structure to PDB (RCSB). Is it possible to submit in RCSD database? Or can only...
07 August 2012 263 7 View
Do we have any on-line servers for docking studies. Please suggest some good on-line servers for protein and ligand minimization.
05 June 2012 5,893 13 View
I have some questions regarding homology modelling i.e, how can I find the protein sequence ,which as already has a structure in PDB?
03 April 2012 1,692 17 View
Can any one help me how to find the complete taxonomy of the vibrionaceae till date any tools in bioinformatics
31 December 2011 6,040 6 View
can we isolate gene from hydrogen-producing bacterial strain ? if so , can we increase the production by changing the sequence I;e modifying
11 December 2011 9,226 0 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.
26 July 2024 7,387 0 View
I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...
22 July 2024 5,953 6 View
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...
17 July 2024 3,785 9 View
I work with planar interdigitated electrodes (IDEs). The finger width and the spacing between the fingers are 5 micron. The surface of the IDEs is coated by a non-conductive polymer layer...
16 July 2024 5,031 1 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View
What is the behavior of anaerobic bacteria in deep salty groundwater with high level of chlorine? Is the nitrate level deceasing and is denitrification controlled by excess chlorine? Thanks
13 July 2024 1,581 4 View