What is the use of whole genome shotgun sequencing ?

09 October 2014 8,964 1 View

Binding capacity of protein?

No interactions but has binding capacity with protein. I have done docking studies and even wet lab experiment . The wet lab experiments showed a good results and not in docking studies like no...

08 September 2013 4,281 3 View

Bacterial-type asparaginases Primers

To amplify the bacterial L-asperginases gene, I need the Primers. Can anyone help me to find a suitable primer sequence and related papers .

10 November 2012 2,156 3 View

Homology modeling

I have a protein with no X ray crystallography structure in PDB database. I am trying to do homology modeling, I found it is showing only 24% with one protein and few are with 30%. My query is,...

08 September 2012 4,809 18 View

Pass predictions

Can any one tell me interpretation of Pass predictions?

08 September 2012 9,269 0 View

RCSB subbmission

A sequence which was retrieved from NCBI - I have modeled the protein structure and now I want to submit the protein structure to PDB (RCSB). Is it possible to submit in RCSD database? Or can only...

07 August 2012 195 7 View

Servers for molecular docking

Do we have any on-line servers for docking studies. Please suggest some good on-line servers for protein and ligand minimization.

05 June 2012 5,811 13 View

Homology modelling of proteins

I have some questions regarding homology modelling i.e, how can I find the protein sequence ,which as already has a structure in PDB?

03 April 2012 1,618 17 View

Taxonomy of vibrionaceae

Can any one help me how to find the complete taxonomy of the vibrionaceae till date any tools in bioinformatics

31 December 2011 5,966 6 View

Brine Shrimp Lethality Assay

Hi every one I want to test the toxicity test of microbial pure compounds and proceed for anticancer activity in cell lines.Can any one tell me BSL (Brine Shrimp Lethality Assay) is good and...

11 December 2011 2,151 5 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View

Does anyone have a copy of the DIBaS dataset?

I am a student from Brunel University London, and I was looking to use the DIBaS dataset for my dissertation. However, recently it has not been accessible via this link:...

26 January 2021 5,185 4 View