What is the use of whole genome shotgun sequencing ?

09 October 2014 8,964 1 View

Can anyone explain the antagonistic activity of bacteria?

When we screen for bio-active compounds producing bacteria we do antagonistic activity. I would like to ask what is the mechanism involved in screening potential bacteria.

09 October 2013 2,662 0 View

Binding capacity of protein?

No interactions but has binding capacity with protein. I have done docking studies and even wet lab experiment . The wet lab experiments showed a good results and not in docking studies like no...

08 September 2013 4,281 3 View

Bacterial-type asparaginases Primers

To amplify the bacterial L-asperginases gene, I need the Primers. Can anyone help me to find a suitable primer sequence and related papers .

10 November 2012 2,156 3 View

Homology modeling

I have a protein with no X ray crystallography structure in PDB database. I am trying to do homology modeling, I found it is showing only 24% with one protein and few are with 30%. My query is,...

08 September 2012 4,809 18 View

Pass predictions

Can any one tell me interpretation of Pass predictions?

08 September 2012 9,269 0 View

RCSB subbmission

A sequence which was retrieved from NCBI - I have modeled the protein structure and now I want to submit the protein structure to PDB (RCSB). Is it possible to submit in RCSD database? Or can only...

07 August 2012 195 7 View

Servers for molecular docking

Do we have any on-line servers for docking studies. Please suggest some good on-line servers for protein and ligand minimization.

05 June 2012 5,811 13 View

Homology modelling of proteins

I have some questions regarding homology modelling i.e, how can I find the protein sequence ,which as already has a structure in PDB?

03 April 2012 1,618 17 View

Taxonomy of vibrionaceae

Can any one help me how to find the complete taxonomy of the vibrionaceae till date any tools in bioinformatics

31 December 2011 5,966 6 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Hi everyone, I want to lower the pH of microbiological broth, what are the best options?

I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.

03 February 2021 6,959 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View

How can I know the stationary phase of growth of several bacteria?

How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...

31 January 2021 4,528 10 View