Which order is right-1 oral presentation 2 manuscript; or 1 manuscript 2 oral presentation ?

I want to present my work as oral presentation in an International congress which they will publish the summary in an International journal. If I publish this study as an full text article in a...

22 June 2018 4,518 2 View

Is it possible to do sequencing after real time PCR or Is there any way to confirm the results of a TaqMan kit for real time PCR?

We are going to do real time PCR with TaqMan SNP Genotyping assay to determine the genotypes of a population. However we donot have a positive control. I am wondering that is it possible to do...

21 December 2016 8,775 6 View

Is it possible to do sequencing after real time PCR or Is there any way to confirm the results of a TaqMan kit for real time PCR?

We are going to do real time PCR with TaqMan SNP Genotyping assay to determine the genotypes of a population. However we donot have a positive control. I am wondering that is it possible to do...

11 December 2016 9,541 6 View

Hba1c result different between boronate affinity and HPLC method?

When comparing HBa1c analyzer in our laboratory we compared 120 patients results with each other? We performed the study in 2 same brand boranate affinity and two HPLC analyzer. All the patients...

21 March 2015 8,779 2 View

How can one use TSP-PRED properly for submitting protein sequences?

15 December 2014 9,501 5 View

Can someone advise on purified his-tag protein in elution buffer containing 500 mM imidazole (His Spin Trap kit - GE Healthcare)?

I eluted purified his-tag protein in 200 ul of elution buffer containing 500 mM imidazole. Then, I want to use it for SDS-PAGE to confirm the presence of specific his-tag protein band on gel. What...

04 August 2013 3,915 11 View

How to trim adaptor / linker sequences from EST sequences before submitting to NCBI?

Any available software for this purpose?

29 July 2013 480 2 View

Does anyone have experience with protein precipitation from yeast cell lysate (Saccharomyces cerevisiae)?

Which method is suitable for precipitating protein from yeast cell lysate? From my own search, I found that the method of precipitating the protein is not a single method because there are a few...

23 July 2013 1,275 5 View

No migration of protein sample from stacking gel to separating gel

What factors are affecting this condition? separating gel- 12% stacking gel- 4% Even my protein marker did not show any bands in separating gel after staining with coomassie blue and destaining...

16 July 2013 3,205 27 View

Does anyone have experience with running protein samples on agarose gel?

Is it possible to run protein samples on agarose gel only?My predicted size of desired proteins is around 30-60 kDa. How much the concentration of agarose should be used for separating protein...

12 July 2013 1,218 9 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

No title

01 March 2021 3,905 5 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View

How do historians determine the credibility sources in modern history?

For a source to be considered reliable it must contain accurate historical information. But how does one determine the accuracy and reliability of sources in modern and recent history since not...

23 February 2021 1,346 13 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

How to do a surface treatment of uncoated TiO2 with polymer?

Basically, I would like to know how to treat or coat on the uncoated bare TiO2 (Ti-OH rich) surface with polymer which has anchoring groups in the polymer structure like trimethoxygroups. What is...

21 February 2021 4,657 6 View

Methodological confusions regarding deductive and inductive reasoning, hypothesis and theory test?

Hi Colleagues- What do you think about the following methodological issues: 1. Is it methodologically wrong to formulate quantitative research questionnaire based on inductive reasoning? 2. Is it...

21 February 2021 4,634 6 View

Where I can find materials to learn Fuzzy VIKOR methodology?

Please suggest ways to learn Fuzzy VIKOR methodology in a best possible way. And is there any toolkit available for the same? Thank you in advance.

20 February 2021 8,271 3 View