How can one use TSP-PRED properly for submitting protein sequences?

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Which order is right-1 oral presentation 2 manuscript; or 1 manuscript 2 oral presentation ?

I want to present my work as oral presentation in an International congress which they will publish the summary in an International journal. If I publish this study as an full text article in a...

22 June 2018 4,518 2 View

Is it possible to do sequencing after real time PCR or Is there any way to confirm the results of a TaqMan kit for real time PCR?

We are going to do real time PCR with TaqMan SNP Genotyping assay to determine the genotypes of a population. However we donot have a positive control. I am wondering that is it possible to do...

21 December 2016 8,775 6 View

Is it possible to do sequencing after real time PCR or Is there any way to confirm the results of a TaqMan kit for real time PCR?

We are going to do real time PCR with TaqMan SNP Genotyping assay to determine the genotypes of a population. However we donot have a positive control. I am wondering that is it possible to do...

11 December 2016 9,541 6 View

Hba1c result different between boronate affinity and HPLC method?

When comparing HBa1c analyzer in our laboratory we compared 120 patients results with each other? We performed the study in 2 same brand boranate affinity and two HPLC analyzer. All the patients...

21 March 2015 8,779 2 View

Can someone advise on purified his-tag protein in elution buffer containing 500 mM imidazole (His Spin Trap kit - GE Healthcare)?

I eluted purified his-tag protein in 200 ul of elution buffer containing 500 mM imidazole. Then, I want to use it for SDS-PAGE to confirm the presence of specific his-tag protein band on gel. What...

04 August 2013 3,915 11 View

How to trim adaptor / linker sequences from EST sequences before submitting to NCBI?

Any available software for this purpose?

29 July 2013 480 2 View

Does anyone have experience with protein precipitation from yeast cell lysate (Saccharomyces cerevisiae)?

Which method is suitable for precipitating protein from yeast cell lysate? From my own search, I found that the method of precipitating the protein is not a single method because there are a few...

23 July 2013 1,275 5 View

No migration of protein sample from stacking gel to separating gel

What factors are affecting this condition? separating gel- 12% stacking gel- 4% Even my protein marker did not show any bands in separating gel after staining with coomassie blue and destaining...

16 July 2013 3,205 27 View

Does anyone have experience with running protein samples on agarose gel?

Is it possible to run protein samples on agarose gel only?My predicted size of desired proteins is around 30-60 kDa. How much the concentration of agarose should be used for separating protein...

12 July 2013 1,218 9 View

Storage temperature for yeast liquid culture (saccharomyces cerevisiae)

What is the suitable temperature for keeping yeast liquid culture after incubating at 30c, 250 rpm for overnight? The type of yeast is saccharomyces cerevisiae. I plan to keep it first for a few...

05 July 2013 9,275 11 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Budak Drop out

need help...

Harinder Singh

Dear Budak, we are currently updating our servers. The problem will be shorted out in few days.

okay,thanks.. I'm interested to use your server. :)

Muralidhar Reddivari

looks like a server issue. hope the admin will sort it out

Dear Budak, we have updated the TSPPRED server and the there is no limit on the length of the protein. You can use it now.