Are there potential hazards/serious adverse effects while dealing with brain tumor tissues in lab?

10 October 2016 0 10K Report

I am planning to work on human brain tumor tissues as part of my research.

I need to know if certain potential hazards are associated with it?

Badges
Science topic

More Sumera Sumera's questions See All

Problems in silver staining of gels?

Hi, I have tried silver staining many times to stain gels, but i dont get the results. After incubating the gels in silver nitrate, i do not get any results.I have changed my silver nitrate too,...

03 April 2018 2,828 4 View

Chemically induced Glioblastoma Animal Model?

Hi, Can anyone advise me about chemically induced GBM model? How valid they are in human GBM research? Thanks

09 October 2017 7,890 1 View

Is there any tool/software etc through which I can search proteins based on its molecular weight?

Hi all, I have got some differentially expressed bands (proteins) on my SDS-PAGE, having molecular weight 40 and 35 kDa. Now I want to find out which proteins are they. Is there any software/tool...

07 August 2017 9,467 0 View

Quantity of proteins from frozen cell lines and fresh culture?

Hi all, I am extracting proteins from cells frozen at -80C. Will it effect the quantity of proteins as compared to the quantity extracted from fresh cell cultures? Thankyou

07 August 2017 8,088 3 View

Bioinformatics tools for proteins?

Hi all, I need to apply some bioinformatics tools on some novel proteins and publish it. Kindly help.

06 July 2017 9,658 3 View

Can anybody check the review I have written for publication?

Hi, I have written a review entitled: ABBERANT GLYCOSYLATION OF CELL ADHESION MOLECULES (CAMs) IN PROGRESSION OF CANCER: HIGHLIGHTING GLIOMAS. Can anybody check it for me before i go for its...

05 June 2017 724 1 View

Lab groups working on proteomic analysis of Glioblastoma Multiforme??

Hi all, I have got a six months research fellowship. I am in search of some relevant labs to work in. My PhD project aims at the identification and characterization of novel altered proteins in...

02 March 2017 10,121 2 View

Lectin Probed Western Blotting?

I need a protocol for Lectin probed western blotting for the analysis of differentially expressed glycoproteins.

31 December 2016 210 1 View

What is the best method for Staining and Destaining the gels in SDS-PAGE?

Hi, Someone please explain me the exact solutions which can stain and destain the gels after SDS-PAGE.

31 December 2016 5,309 11 View

Protein Extraction from U87 MG cell lines?

Hi, Previously, I had done protein extraction from tissue (mice brian). Now I need to extract proteins from cell lines (U87 MG). Can i follow the same protocol? If someone has a protocol or...

31 December 2016 6,651 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Project/Experimental assistance ？?

Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...

05 August 2024 5,829 1 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

Short Synthesis of Graphene Oxide from Natural Graphite Flakes?

I am in search of a modified Hummer's method, which can be used to synthesis graphene oxide within 6-8 hours. As I am a student it is not allowed for us to work after 5 in the laboratory. So I am...

01 August 2024 8,368 2 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View