Why do some full text requests not allow me to respond to the requesting person?

Over the last year, when I have clicked on 'View the Request' in the notifying e-mail for a full text request, I have been taken to the paper in question but there is no request visible and thus I...

16 January 2021 7,558 1 View

How can I accelareate dehydration of mouse lung with 10% sucrose for frozen section?

09 August 2020 4,967 1 View

How to visualize output files of Quantum Espresso?

I am a starter of Quantum Espresso. I want to visualize the output files from Quantum Espresso. Primarily, I want to see atom configurations and trajectories from .xyz files and .path files....

20 June 2020 8,755 1 View

Can anyone help me about errors of Process Macro multiple mediator analysis with model 4?

When I start PROCESS I get two error messages: ->Error 12410 Source operand for EVAL is not balanced. > >Undefined error #12354 - not found in file .. How can I solve this...

26 March 2020 6,738 2 View

How many mice are sacrificed to obtain 1 mg antibody with the ascites method?

To get an idea about the animal welfare impact of my planned experiments and to perhaps make adjustments in the antibody selection, I was wondering: How many mice are sacrificed to obtain 1 mg...

08 March 2020 2,350 3 View

Which MCDA-method (and/or software) is most suitable for a decision with alot of scenarios?

For my thesis, which thematizes a GIS-based industrial site selction in Europe, i am in need of a method to integrate all of the european NUTS 2-regions (253 units). The methods which i had in...

29 February 2020 6,743 5 View

Why doesn't my iron crystal format an isosceles triangular twining boundary?

I have simulated iron crystal (BCC) in Lammps using many different potential files in order to make an isosceles TB. I modeled the TB by rigorously calculated the offset distances of the two...

02 September 2019 9,403 1 View

LCMS microcolumns: POROS 50 Reversed R1 vs R2?

I am looking for material to make my own microcolumns/ in-tageTips for peptide purification before LCMS-analysis. Earlier i have used C18-disc and POROS 50 R2 reversed powder (column material)....

31 July 2019 1,453 4 View

RET immunohistochemistry in NSCLC?

Can someone give me a recommendation for a RET antibody for NSCLC diagnostics to detect a ret gene rearrangement? Is there something like a mutation-specific antibody in the market? for...

01 May 2018 9,827 2 View

'First Principle Reasoning' / 'Physics Thinking' for radical innovation - will it emerge as a viable research area?

Is anybody interested in or already doing research within the field of 'First Principle Reasoning' / 'Physics Thinking' for radical innovation? It's a mental model entrepreneur Elon Musk is...

02 April 2018 7,616 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

Are there any exception like work hardening in metals elastic limit due to dynamic loading?

The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...

11 February 2021 952 6 View

How to correctly reference instruments in systematic review following APA?

Hello, I am running into an issue and do not know how to cite / reference correctly here. APA states that instruments and measurements need to be referenced with the author's name. At the same...

11 February 2021 9,523 3 View

How to modify micro-structure of Pure Copper which is available as a flat plate of 25 X 25 sq. cm?

Hi everyone, for a thesis project I have to modify the micro-structural properties of pure Cu flat plate of dimension 25 X 25 sq. cm . As I tried searching the available literature, I found a lot...

09 February 2021 5,090 3 View