Hello!
I have worked with many cancer lines that carry various mutations and gene rearrangements. But now I have a question - are there stable (or, with a lifespan limited by the natural Hayflick limit) cell lines derived from patients with, for example, Down syndrome or Shereshevsky-Turner syndrome?
Hello Dmitry Lifanov
Yes, there is the human fibroblasts Detroit -532 (a human Down syndrome cell line, ATCC CCL-54).
You may maintain this cell line in DMEM High glucose supplemented with 10 % FBS and 1 % non-essential amino acids.
FYI: A longevity study performed by ATCC showed that the depositor's material was capable of growing for an additional 28 population doublings (i.e., unknown + 28) before the onset of senescence.
Best.
Yes, there are stable cell lines with chromosomal number abnormalities, including those derived from patients with Down syndrome and Turner syndrome. These cell lines are often used in scientific research to study the effects of chromosomal abnormalities on cellular behavior and to develop new treatments for diseases associated with these conditions.
Down syndrome, also known as trisomy 21, is caused by an extra copy of chromosome 21. There are several stable cell lines that have been established from individuals with Down syndrome, including fibroblast cell lines such as GM04897 and GM13113A, which were developed from skin biopsies. These cell lines have been used to study the effects of trisomy 21 on cellular processes such as gene expression, cell growth, and apoptosis.
Turner syndrome, also known as monosomy X, is caused by a missing or partially deleted X chromosome. There are also stable cell lines that have been established from individuals with Turner syndrome, including fibroblast cell lines such as TS01 and TS02, which were developed from skin biopsies. These cell lines have been used to study the effects of monosomy X on cellular processes such as gene expression, cell growth, and apoptosis.
Other examples of stable cell lines with chromosomal number abnormalities include cell lines derived from individuals with Klinefelter syndrome (XXY), Triple X syndrome (XXX), and XXYY syndrome.
It's worth noting that while these cell lines are stable and can be cultured indefinitely, they do have a limited lifespan due to the Hayflick limit, which is the maximum number of times a normal human cell can divide before entering senescence. However, some cell lines can be immortalized through techniques such as viral transformation or expression of telomerase, allowing them to continue dividing beyond the Hayflick limit.
Overall, stable cell lines with chromosomal number abnormalities are valuable tools for studying the effects of chromosomal abnormalities on cellular behavior and for developing new treatments for diseases associated with these conditions.
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