Why do we wash azolectin with acetone?

I want to prepare liposomes made of azolectin (sometimes spelled as asolectin). I start with the azolectin powder, and many protocols say that I should wash it with acetone (dissolve, centrifuge,...

04 February 2021 2,738 3 View

How to solve 1-D Wave equation?

Good day, In standard PDE literature, there are a lot of solution approaches for the 1-D wave equation initial-boundary value problem. One way is with separation of variables. Below I describe...

25 January 2021 4,876 3 View

Journal Quartiles for Research Evaluation?

Dear all, Could you please tell me the countries where the journal quartiles SJR/JCR are used for the state system for research evaluation (except for Russia)? Best, Dmitry

21 June 2020 5,635 7 View

Could you please suggest an XRD data viewer?

Hi. I'd appreciate if someone could suggest a powder XRD (NPD, PDF as an option) data viewer which: (1) Is free of charge (2) Is easy to operate, i.e. can be run with a double-click, multiple...

06 June 2020 6,493 2 View

Could my SP2/0-Ag14 be cross-contaminated?

Hello! Currently I propagating my SP2/0 for hybridoma. It's a first time when I working with this line. According to ATCC this line must grow in suspension, but when I'm doing my daily observation...

20 March 2020 5,191 2 View

How we can painting with powder by electrostatic method without Faraday effect?

Good day, dear colleagues I have some question for You I'm working on the powder painting of details with difficult geometry. The tribostatic method has a good result about this I try to use...

25 February 2020 2,190 3 View

Is there any sources for 661-W cell line?

Hello! For my current project I need cell line 661-W - this is a mouse ganglion precursor-like cells. I had checked many supplies (ATCC, Sigma Aldrich etc) - but none offers this line. I even...

03 February 2020 1,011 2 View

Manuscripts for the 4th volume of the "Biodiversity, biogeography and nature conservation in Wallacea and New Guinea"?

26 November 2019 1,452 4 View

Where to go to the conference?

20 October 2019 6,938 3 View

Can I leave slides in Ventana overnight after finishing of staining program?

Hello everyone! On my previous job I worked with Ventana Benchmark Ultra and performed various IHC stainigs on this device. As far as I can remember, engineer said, that after finishing of...

21 February 2019 3,193 2 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Can polymer concentrations be calculated based on the number average molecular weight?

Hello, I spin-cast two different polymers onto wafers and measure their height as a function of initial concentration. For polymer A, I have the weight-average molecular weight (Mw) as well as...

28 February 2021 7,882 2 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View