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Questions related from Dmitry Lifanov
Hello! I have a question regarding transfection stability in MCF7 and IMR32. For my experiment I need to transfect them with a plasmid with my gene of interest and Kan/Neo-gene which confers...
03 December 2023 1,582 2 View
Hello! I have worked with many cancer lines that carry various mutations and gene rearrangements. But now I have a question - are there stable (or, with a lifespan limited by the natural Hayflick...
14 August 2023 565 2 View
Hello! I'm a little bit confused - all of my life I was taught, that cells must be preserved in a mixture of 90% FBS + 10% DMSO (maybe 5% EG + 5% DMSO, for stem cells). But right now I'll have to...
06 November 2022 5,269 6 View
Hello! I rarely worked with ADCC-assays, but in different protocols I met a mentions of different cells types: 1) PBMC's (ficoll-purified, without any further separation) 2) NK-cells, purified...
07 March 2022 4,670 2 View
Hello! Currently I propagating my SP2/0 for hybridoma. It's a first time when I working with this line. According to ATCC this line must grow in suspension, but when I'm doing my daily observation...
21 March 2020 5,334 2 View
Hello! For my current project I need cell line 661-W - this is a mouse ganglion precursor-like cells. I had checked many supplies (ATCC, Sigma Aldrich etc) - but none offers this line. I even...
04 February 2020 1,244 2 View
Hello everyone! On my previous job I worked with Ventana Benchmark Ultra and performed various IHC stainigs on this device. As far as I can remember, engineer said, that after finishing of...
22 February 2019 3,396 2 View
