Are you equipped to count small plasmid chromosomes?

I wish to perform an experiment which will provide support for a novel DNA structure. Most of the experiment is conceptually simple, easy to perform, and inexpensive. But the key to doing it is...

29 November 2020 5,258 9 View

How to plot gaussian distribution on Matlab?

15 May 2020 9,150 3 View

Do you know freeware and/or methodology which can do the interference fringe visibility curve fitting?

23 February 2020 6,176 3 View

Negative % degree of hydrolysis values for OPA assay?

Hi, I am using the o-phthalaldehyde assay (OPA) to quantify the % degree of hydrolysis in bacterial fermented milk supernatants. However, for some of my isolates I am obtaining negative %DH...

22 February 2018 2,459 3 View

I am attempting to preserve the shelf life of ice tea up to six months with citric acid, I need a perfected ratio?

Any one who can help with a ratio that can preserve 3-6months of packed ice tea,without increasing the pH

14 November 2017 229 1 View

How many strains are needed to test for selective/differential media?

Hey, does any one know how many strains are needed to test before a selective/differential media can be confirmed as selective/differential for a particular microorganism?

12 January 2016 7,849 3 View

When choosing selective media to isolate enterococci, is it better to use kanamycin aesculin azide agar or bile aesculin agar?

Is it better to use kanamycin aesculin azide agar or bile aesculin agar when selecting for enterococci? Are there advantages of one media over the other? Thanks

26 October 2015 3,722 3 View

How best to measure nitric oxide in cultured cells?

03 April 2015 3,760 8 View

Platinum migration at high temperatures

What factors (in addition to temperature) contribute to the migration of platinum in Pt thin films at high temperatures? What methods are there to prevent or inhibit this from happening?

27 March 2013 1,829 3 View

Why do inverted organic cells typically have lower efficiencies than the standard architecture? ?

Why do inverted organic solar cells typically have lower efficiencies than the standard architecture? Wikipedia says this as do a few other places but I cannot find an explanation, the references...

01 January 1970 219 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View