Are you equipped to count small plasmid chromosomes?

I wish to perform an experiment which will provide support for a novel DNA structure. Most of the experiment is conceptually simple, easy to perform, and inexpensive. But the key to doing it is...

29 November 2020 5,258 9 View

What is the best way of on-boarding in-service teachers in ICT integration in teaching and learning in developing countries?

Many in-service teachers were not trained on ICT integration. COVID 19 has made it clear that teachers need to have been well versed with the use of ICT in teaching and learning environment. This...

27 September 2020 4,646 1 View

How to plot gaussian distribution on Matlab?

15 May 2020 9,150 3 View

Do you know freeware and/or methodology which can do the interference fringe visibility curve fitting?

23 February 2020 6,176 3 View

Negative % degree of hydrolysis values for OPA assay?

Hi, I am using the o-phthalaldehyde assay (OPA) to quantify the % degree of hydrolysis in bacterial fermented milk supernatants. However, for some of my isolates I am obtaining negative %DH...

22 February 2018 2,459 3 View

Are there companies still performing 454-pyrosequencing?

Since this technique is optimized for my samples, and I need to compare with previous results also done with 454, I am hoping to still find some companies performing this analysis (since today...

15 September 2016 836 3 View

How many strains are needed to test for selective/differential media?

Hey, does any one know how many strains are needed to test before a selective/differential media can be confirmed as selective/differential for a particular microorganism?

12 January 2016 7,849 3 View

When choosing selective media to isolate enterococci, is it better to use kanamycin aesculin azide agar or bile aesculin agar?

Is it better to use kanamycin aesculin azide agar or bile aesculin agar when selecting for enterococci? Are there advantages of one media over the other? Thanks

26 October 2015 3,722 3 View

How best to measure nitric oxide in cultured cells?

03 April 2015 3,760 8 View

Platinum migration at high temperatures

What factors (in addition to temperature) contribute to the migration of platinum in Pt thin films at high temperatures? What methods are there to prevent or inhibit this from happening?

27 March 2013 1,829 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

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22 February 2021 9,932 11 View