Are you equipped to count small plasmid chromosomes?

I wish to perform an experiment which will provide support for a novel DNA structure. Most of the experiment is conceptually simple, easy to perform, and inexpensive. But the key to doing it is...

29 November 2020 5,258 9 View

How to plot gaussian distribution on Matlab?

15 May 2020 9,150 3 View

Do you know freeware and/or methodology which can do the interference fringe visibility curve fitting?

23 February 2020 6,176 3 View

What are typical methods of producing HEA's?

I've been curious about how exactly HEA's are manufactured. Are they predominately cast? Printed with additive manufacturing?

29 December 2019 6,887 2 View

Is there a known method for the inhibition of aluminosilicate gel formation at high pH e.g. chelation of aluminium?

Looking for a method to create a solution containing dissolved aluminium and silicate but without the gel formation.

04 July 2019 3,683 1 View

Qiagen Blood & Tissue Kit with plants?

I know Qiagen makes plant specific extraction kits, but has anyone tried the DNeasy blood and tissue kit with plant material? Thanks, Matt

24 March 2019 8,520 2 View

Are there theoretical limits to how many viable T cells can be derived from peripheral blood donation (e.g., PBMCs) of a single healthy donor?

Immune oncology drug programs are increasingly moving towards use of allogeneic T cells which can be engineered and used with unmatched recipients. Celyad, for instance, recently got clearance...

15 August 2018 1,122 2 View

Can isoelectric points of my protein affect nickel affinity chromatography?

When purifying my his-tagged protein much of it was eluting at only 50mM imidazole. The protein was binding to the charged beads as shown by samples from the beads and supernatant after incubation...

30 March 2018 5,096 4 View

Negative % degree of hydrolysis values for OPA assay?

Hi, I am using the o-phthalaldehyde assay (OPA) to quantify the % degree of hydrolysis in bacterial fermented milk supernatants. However, for some of my isolates I am obtaining negative %DH...

22 February 2018 2,459 3 View

How long does it generally take to recieve the full-text article/journal?

I have made several requests this afternoon for full-text articles/Journals. I am a new user to this site and am just curious how long it generally takes to process those requests. Thank you!

11 December 2017 4,893 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View