Are there any reporter assays for functionality test of miRNAs targeting in the coding region or the 5'UTR regions of the respective mRNA target??

More Megha Sravani Bondada's questions See All

Reason for discontinuities in my Band structure?

Hey All! I am wondering what might be wrong with my band structure. I did the calculations using VASP and plotted the results using Origin. Although I have tried changing various input...

25 July 2024 2,920 11 View

Can anyone suggest some monoembryonic rootstock of mango with abiotic or biotic resistance?

17 April 2024 1,568 1 View

How to get a smoother DOS plot in VASP?

Hello All! I am hereby attaching the DOS plot for my system. As you can see, it has too many spikes., however I want a smoother plot. I have used ISMEAR = -5 and NEDOS = 3000. Do I need to make...

19 March 2024 4,457 4 View

Why adonitol or ribitol is used as a internal standard in GC-MS untargeted metabolomics of plant?

26 December 2023 6,148 0 View

Which is the best medium to store extracted deciduous/primary teeth for research purpose over a long period of time (6-12months or more)?

Many in-vitro studies are done on primary teeth, especially to study their internal structure. We have to store a sufficient number of teeth before we start our study. Which solution would be the...

21 December 2023 5,693 4 View

Which Indices are suitable to assess the gingival health in primary and mixed dentition for pediatric population?

To assess the gingival health in pediatric population, what indices are recommended?

07 August 2023 9,704 7 View

What are index teeth in Gingival Index by Loe and Silness (1963) for assessment in Primary and Mixed dentition??

The Gingival Index by Loe and Sillness (1963) is widely being used in epidemiological studies to assess the Gingival health. The modified scale assesses 6 index teeth in the permanent dentition to...

07 August 2023 3,713 3 View

What are key points when performing Arabidopis leaf tissue staining with FM4-64 dye?

I want to study endocytic pathway for a specific plant protein using FM4-64 dye and leaf tissue from A. thaliana transgenic plants. Most of the studies were performed using only root tissues. Have...

01 May 2023 5,306 0 View

Has anyone done a PES Scan in gaussian using Centroid defined by GIC?

I am trying to do a potential energy scan of a BTA (N,N',N''-trismethyl-benzene-1,3,5-tricarboxamide) dimer. I defined the coordinates of the COM (I choose 6 carbons of benzene ring for this)...

30 April 2023 557 0 View

Does anyone check the expression of GABARAP protein in GBM cell line ( U87MG) ?

My westerns haven't worked for the past three to four months, which is an issue. I attempted using two different antibodies from two different companies, but I got no blots. I load 50 to 100 ug of...

22 April 2023 1,331 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

Assistance with MTT Assay Data ?

I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...

26 July 2024 7,511 3 View