What if top fiber concrete strain exceeds the ultimate strain when fixing the yield strain in steel i.e to calculate yield moment in circular column?

I have prepared an excel sheet to calculate yield moment and curvature for a hollow circular section. In which Depth of Neutral Axis is fixed using "GOAK SEEK" function. Axial Force is input of...

22 November 2020 4,632 3 View

Should I perform transcardial perfusion by PBS before lung isolation for RNA expression analysis?

Hey, I am planning to dissect rats and isolate Lung tissue samples to be stored in RNA stabilizer for later analysis. - Should I perform trandcardial perfusion by PBS ? - Is it necessary to...

21 September 2020 1,822 5 View

ELISA of isomer of glycolytic intermediate 2,3 DPG ?

Hello, I recently performed Kit based ELISA for 2,3 DPG on plasma samples of control and hypoxia treated rats. [CAT. NO. E1562Ra, Bioassay technology laboratory] However, the results I obtained...

07 September 2020 3,270 2 View

Can I use one facet of a multidimensional latent variable questinaairre/scale?

I wish to use self-monitoring scale developed by Richard D. Lennox and Raymond N. Wolfe ("Revision of self-monitoring"). It is a 2 factor structure scale with total 13 items- (1) ability to modify...

17 May 2020 8,027 3 View

Can any researcher suggest the relation of NMR and XRD analysis?

need to relate both in my future research

26 April 2020 6,176 3 View

Corona virus testing ?

we have a nano formulation that needs to tested against COVID19. INVITRO in a lab. Does anybody know where it can be tested Dr Ragahvan

31 March 2020 200 9 View

On column TEV cleavage?

Hi, I have cloned my protein with TEV protease site (His tag-TEV site-linker-protein). I purify my protein with Ni-NTA and then do on column TEV cleavage (overnight). I hardly see any TEV...

10 March 2020 1,205 3 View

How to get a good SDS page gel for 16KDa protein?

I want to see a 16kDa band of my protein in SDS PAGE. Whenever i run the gel, the band in the upper part of resolving gel were very sharp and clear however the lower part where i supposed to get...

11 February 2020 7,749 2 View

What is current state of machine learning in the field of condition monitoring?

I am looking for the possible implementation of ML tools for electrical machines. If any of the approach has even been used in the industry?

28 January 2020 8,165 3 View

Any Suggestions to rectify negative intercept in HPLC?

Drug is Sodium citrate, diluting drug in de-ionized water. Using a C18 column. the concentration range of 1000-3000microgram to lower range of 25-300microgram. Thanks!

19 September 2019 7,315 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View

How to reduce residual stresses in MD simulation?

I am working on a preparation of representative polymeric structures of some popular polymers, such as polyimide or PTFE for MD simulation, during which a single nitrogen or oxygen atom will be...

24 February 2021 8,105 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View