Are there any proteins that can not be denatured by Urea or Guanidium HCL ? If SO can anyone give me an example ??
Following☺ Omar A. Abdulqader
one strand - SSDNA - BIOTIN LABELLED another strand - SSDNA - NO LABEL without using NaCl , for Bli experiment ?
27 November 2023 9,035 0 View
How to Distinguish between DH5 alpha and BL21 cells if they are not labelled ?
25 August 2023 7,001 0 View
can you plese suggest other ways of restriction digestion of DNA.
19 June 2023 7,900 3 View
How can the size of protein (6kda) , dna (18bp, app. 6kda size ) and resultant complex around 12kda effects the change in the Fluorescence anisotropy ? Is there any correlation between the size...
01 April 2023 894 1 View
I am working on SSR markers.I have used Genodive software for analysis of tetraploid data. To check the genetic differentiation in 12 populations I applied AMOVA and the result file has attached....
11 April 2022 7,186 2 View
I don't know what happened. Suddenly I am unable to find my previous runs (DLS and zetapotential) data in my dts file. Even .del file was also not created which means data was not deleted. Anybody...
02 February 2022 9,008 5 View
can we use a palindromic sequence having dsDNA labelled with cyanine fluorophores for fret experiments ?
05 October 2021 5,629 1 View
y = A1*exp(-x/t1) + A2*exp(-x/t2)+y0
14 July 2021 7,668 3 View
What are the recommended buffers and ionic strengths for pH 10 , pH 12, pH 14, to study protein secondary structure in circular dichroism ?
04 June 2021 7,942 4 View
Can any one suggest me concentration of beta-mercaptoethanol (2-ME) or DTT to prevent cysteine dimerisation in protein purification at pH 8.0 . and stability of them in Tris Hcl buffer with...
16 December 2019 3,160 4 View
I want to remove Urea from my protein (6kDa) sample. I did use : 1. Step down the Urea gradient (dialysis) in order to remove urea and refold my protein, but my protein still contains urea in the...
06 August 2024 1,350 4 View
I have a 0.25mM thick gel and I am having trouble cleaning out the wells to ensure constant sample application. I usually use a thin syringe to get into the wells and rinse them out with running...
01 July 2024 2,124 2 View
Some scientists use nano nitrogen and some use nano urea, even though the process used the same for preparation. So, which is true nano nitrogen or nano urea?
28 June 2024 2,885 6 View
Fertilizers play a crucial role in enhancing the productivity of plants. However, low nutrient use efficiencies of conventional fertilizers (CFs) associated with several losses have led to...
21 June 2024 8,843 3 View
Hello, I am trying to purify a 6X-His tagged protein using Ni-NTA chromatography. However, I obtain very less yield & concentration; and considerable protein in load flow through. Any...
16 June 2024 5,876 5 View
Urea fertilizer concentration percentage of nitrogen
07 June 2024 488 5 View
Hi everyone, I've been having difficulty in purifying a protein. It is a beta barrel, integral membrane protein. My protein is expressed and purified recombinantly. My target protein is running...
30 May 2024 7,604 3 View
Need a simple and small scale preparation methods
23 May 2024 3,843 0 View
Can I ask for a JCPDS card no. 01-083-1436 or CPDS file number 00-031-1979? I need to index a urea derivative compound to the face-centered tetragonal structure, JCPDS card no....
15 May 2024 9,751 2 View
I prepared h-BN by hydrothermal methods by boron anhydride and urea after hydrothermal I was heated at 800 temps for 12 hours after that my sample stuck to the crucible the third time, and the...
15 May 2024 7,354 0 View